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Literature summary for 2.7.7.88 extracted from

  • Neubauer, J.; Ogino, M.; Green, T.J.; Ogino, T.
    Signature motifs of GDP polyribonucleotidyltransferase, a non-segmented negative strand RNA viral mRNA capping enzyme, domain in the L protein are required for covalent enzyme-pRNA intermediate formation (2016), Nucleic Acids Res., 44, 330-341.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
F1269A residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells vesicular stomatitis virus
G1100A residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells vesicular stomatitis virus
G1154A residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation and also reduces GTPase activity. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells vesicular stomatitis virus
P1104A residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells vesicular stomatitis virus
P1104V residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells vesicular stomatitis virus
Q1270A residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells vesicular stomatitis virus
T1157A residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells vesicular stomatitis virus
T1157S residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells vesicular stomatitis virus
W1188A residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation and also reduces GTPase activity. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells vesicular stomatitis virus
Y1152A residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation and also reduces GTPase activity. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells vesicular stomatitis virus
Y1152W residue is essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation and also reduces GTPase activity. Mutant induces termination of mRNA synthesis at position +40 followed by aberrant stop-start transcription, and abolishes virus gene expression in host cells vesicular stomatitis virus

Organism

Organism UniProt Comment Textmining
vesicular stomatitis virus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pppAACAG + GDP oligo-RNa substrate. Residues G1100 in motif A, T1157 in motif B, W1188 in motif C, and F1269 and Q1270 in motif E are essential or important for the PRNTase activity in the step of the covalent L-pRNA intermediate formation, but not for the GTPase activity that generates GDP vesicular stomatitis virus diphosphate + guanosine 5'-pppAACAG
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