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Literature summary for 2.7.7.86 extracted from

  • Kato, K.; Ishii, R.; Goto, E.; Ishitani, R.; Tokunaga, F.; Nureki, O.
    Structural and functional analyses of dna-sensing and immune activation by human cGAS (2013), PLoS ONE, 8, e76983.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta2 (DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 18% (w/v) PEG3350, 0.2 M ammonium nitrate, 0.5 M NaCl, and 0.02 mM CYMAL-7 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + GTP Homo sapiens
-
2 diphosphate + cyclic Gp(2'-5')Ap(3'-5')
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8N884
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GTP
-
Homo sapiens 2 diphosphate + cyclic Gp(2'-5')Ap(3'-5')
-
?

Synonyms

Synonyms Comment Organism
cGAS
-
Homo sapiens
cyclic GMP-AMP synthase
-
Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme induces the activation of STING by producing cGAMP from GTP and ATP Homo sapiens