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Literature summary for 2.7.7.85 extracted from

  • Zarrella, T.M.; Yang, J.; Metzger, D.W.; Bai, G.
    Bacterial second messenger cyclic di-AMP modulates the competence state in Streptococcus pneumoniae (2020), J. Bacteriol., 202, e00691-19 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.85

Protein Variants

Protein Variants Comment Organism
V76G by introducing a V76G mutation in CdaA, a cdaA* strain is constructed, which produces lower levels of c-di-AMP, phenotype, overview. Inability of the cdaA* strain to grow in medium with CSP, which is restored by deletions of the genes needed for K+ uptake in Streptococcus pneumoniae. The cdaA* strain prefers media at pH 6.5-7.0 and shows growth arrest at pH 8.0, which is the best growth pH for the wild-type strain. competence-stimulating peptide (CSP) is detrimental to low-c-di-AMP-producing bacteria through K+ uptake by CabP and TrkH Streptococcus pneumoniae serotype 2 D39

Inhibitors

Inhibitors Comment Organism Structure
competence-stimulating peptide CSP, reduces the growth of the wild-type strain by 9% and the growth of the cdaA* V76G mutant strain by 94% compared to without CSP Streptococcus pneumoniae serotype 2 D39

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptococcus pneumoniae serotype 2 D39

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 ATP Streptococcus pneumoniae serotype 2 D39
-
 2 diphosphate + cyclic di-3',5'-adenylate
-
 ?
2 ATP Streptococcus pneumoniae serotype 2 D39 NCTC 7466
-
 2 diphosphate + cyclic di-3',5'-adenylate
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae serotype 2 D39 A0A0H2ZPG9
-
-
Streptococcus pneumoniae serotype 2 D39 NCTC 7466 A0A0H2ZPG9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP
-
 Streptococcus pneumoniae serotype 2 D39 2 diphosphate + cyclic di-3',5'-adenylate
-
 ?
2 ATP
-
 Streptococcus pneumoniae serotype 2 D39 NCTC 7466 2 diphosphate + cyclic di-3',5'-adenylate
-
 ?

Synonyms

Synonyms Comment Organism
CdaA
-
 Streptococcus pneumoniae serotype 2 D39
dacA
-
 Streptococcus pneumoniae serotype 2 D39

General Information

General Information Comment Organism
malfunction a strain that possesses a V76G variation in CdaA produced less c-di-AMP and is highly susceptible to competence-stimulating peptide (CSP). Deletion of cabP and trkH restores the growth of these bacteria in medium with CSP. Peptide and amino acid transport systems are detrimental in c-di-AMP-deficient strains Streptococcus pneumoniae serotype 2 D39
physiological function Streptococcus pneumoniae encodes an essential diadenylate cyclase, CdaA, c-di-AMP is produced by diadenylate cyclase, CdaA, and cleaved by phosphodiesterases Pde1 and Pde2. c-di-AMP binds a transporter of K+ (Trk) family protein, CabP, which subsequently halts K+ uptake via the transporter TrkH. The c-di-AMP-binding protein, CabP, is a mediator of potassium uptake and c-di-AMP homeostasis. c-di-AMP homeostasis must be maintained for competence program integrity. A DELTApde1 DELTApde2 strain exhibits reduced transformation efficiency compared to wild-type bacteria, which is c-di-AMP dependent. Transformation efficiency was also directly related to the [K+] in the medium, suggesting a link between c-di-AMP function and the pneumococcal competence state. Role for c-di-AMP in the competence program of Streptococcus pneumoniae. c-di-AMP affects the pneumococcal transcriptome, and c-di-AMP affects transformation, responsiveness to CSP, and growth under stress conditions, detailed overview. c-di-AMP influences the competence program of Streptococcus pneumoniae partly through modulating K+ uptake. c-di-AMP signaling can mediate pneumococcal survival under several environmental conditions, including differential pH and glycine concentrations Streptococcus pneumoniae serotype 2 D39