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Literature summary for 2.7.7.85 extracted from
- Highly efficient enzymatic preparation of c-di-AMP using the diadenylate cyclase DisA from Bacillus thuringiensis (2013), Enzyme Microb. Technol., 52, 319-324.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of the C-terminal His-tagged protein in Escherichia coli | Bacillus thuringiensis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | the enzyme is strictly dependent on divalent cations, activity in presence of 10 mM Ca2+ is 10.6% compared to activity in presence of 10 mM Mg2+ | Bacillus thuringiensis |  |
| Mg2+ | the enzyme is strictly dependent on divalent cations, optimal activity at 10 mM Mg2+ | Bacillus thuringiensis | |
| Mn2+ | the enzyme is strictly dependent on divalent cations, activity in presence of 10 mM Mn2+ is 13.7% compared to activity in presence of 10 mM Mg2+ | Bacillus thuringiensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41100 | - |
x * 41100, calculated from sequence | Bacillus thuringiensis |
| 43000 | - |
x * 43000, SDS-PAGE | Bacillus thuringiensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus thuringiensis | D5TM67 | - |
- |
| Bacillus thuringiensis BMB171 | D5TM67 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus thuringiensis |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Bacillus thuringiensis |
| ? | x * 41100, calculated from sequence | Bacillus thuringiensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| disA | - |
Bacillus thuringiensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
the enzyme exhibits the highest activity at 55°C for 30 min | Bacillus thuringiensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 65 | 45°C: about 65% of maximal activity, 65°C: about 30% of maximal activity | Bacillus thuringiensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
loss of activity above | Bacillus thuringiensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Bacillus thuringiensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 10 | pH 8.5: about 50% of maximal activity, pH 10.0: about 40% of maximal activity, CHES buffer | Bacillus thuringiensis |
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Release 2023.1 (January 2023)
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