BRENDA - Enzyme Database
show all sequences of 2.7.7.82

The CMP-legionaminic acid pathway in Campylobacter: Biosynthesis involving novel GDP-linked precursors

Schoenhofen, I.; Vinogradov, E.; Whitfield, D.; Brisson, J.; Logan, S.; Glycobiology 19, 715-725 (2009) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
His-tagged protein expressed in Escherichia coli
Campylobacter jejuni
Organism
Organism
UniProt
Commentary
Textmining
Campylobacter jejuni
-
-
-
Campylobacter jejuni 11168
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
immobilized metal ion affinity chromatography (Ni2+)
Campylobacter jejuni
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
CTP + N,N'-diacetyllegionaminic acid
-
719563
Campylobacter jejuni
CMP-N,N'-diacetyllegionaminic acid + diphosphate
-
-
-
?
CTP + N,N'-diacetyllegionaminic acid
-
719563
Campylobacter jejuni 11168
CMP-N,N'-diacetyllegionaminic acid + diphosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * about 30000, His-tagged protein, SDS-PAGE
Campylobacter jejuni
Synonyms
Synonyms
Commentary
Organism
CMP-legionaminic acid synthetase
-
Campylobacter jejuni
legF
-
Campylobacter jejuni
Cloned(Commentary) (protein specific)
Commentary
Organism
His-tagged protein expressed in Escherichia coli
Campylobacter jejuni
Purification (Commentary) (protein specific)
Commentary
Organism
immobilized metal ion affinity chromatography (Ni2+)
Campylobacter jejuni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
CTP + N,N'-diacetyllegionaminic acid
-
719563
Campylobacter jejuni
CMP-N,N'-diacetyllegionaminic acid + diphosphate
-
-
-
?
CTP + N,N'-diacetyllegionaminic acid
-
719563
Campylobacter jejuni 11168
CMP-N,N'-diacetyllegionaminic acid + diphosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * about 30000, His-tagged protein, SDS-PAGE
Campylobacter jejuni
Other publictions for EC 2.7.7.82
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719563
Schoenhofen
The CMP-legionaminic acid path ...
Campylobacter jejuni, Campylobacter jejuni 11168
Glycobiology
19
715-725
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718850
Glaze
Biosynthesis of CMP-N,N-diacet ...
Legionella pneumophila, Legionella pneumophila ATCC 33152D
Biochemistry
47
3272-3282
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-