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Literature summary for 2.7.7.81 extracted from

  • Schoenhofen, I.C.; McNally, D.J.; Brisson, J.; Logan, S.M.
    Elucidation of the CMP-pseudaminic acid pathway in Helicobacter pylori: synthesis from UDP-N-acetylglucosamine by a single enzymatic reaction (2006), Glycobiology, 16, 8C-14C.
    View publication on PubMed
Search for more literature for 2.7.7.81

Application

Application Comment Organism
medicine Helicobacter pylori, heavily modifies its flagellin with pseudaminic acid. Because this sugar is unique to bacteria, its biosynthetic pathway offers potential as a novel therapeutic target Helicobacter pylori

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for optimal activity Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid Helicobacter pylori CMP-pseudaminic acid biosynthesis. Helicobacter pylori, heavily modifies its flagellin with pseudaminic acid, a modification that influences pathogenesis through bacterial adhesion, invasion, and immune evasion diphosphate + CMP-5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid
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 ?

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid CMP-pseudaminic acid biosynthesis. Helicobacter pylori, heavily modifies its flagellin with pseudaminic acid, a modification that influences pathogenesis through bacterial adhesion, invasion, and immune evasion Helicobacter pylori diphosphate + CMP-5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid
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 ?
CTP + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid when all six CMP-pseudaminic acid biosynthetic enzymes (PseB, PseC, PseH, PseG, PseI, PseF) are combined in a reaction with UDP-N-acetylglucosamine, synthesis of VII is successful CMP-pseudaminic acid. Combining the first five enzymes (PseB, PseC, PseH, PseG, PseI) in a one-pot reaction results in 100% conversion of UDP-N-acetylglucosamine to pseudaminic acid after 20 h Helicobacter pylori diphosphate + CMP-5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid
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 ?

Synonyms

Synonyms Comment Organism
PseF
-
 Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 alkaline pH is required for optimal activity Helicobacter pylori