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Literature summary for 2.7.7.8 extracted from

  • Numata, S.; Nagata, M.; Mao, H.; Sekimizu, K.; Kaito, C.
    CvfA protein and polynucleotide phosphorylase act in an opposing manner to regulate Staphylococcus aureus virulence (2014), J. Biol. Chem., 289, 8420-8431.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 RNA(n) pH 8.4, 37°C Staphylococcus aureus
0.042
-
 2',3'-cyclic RNAn pH 8.4, 37°C Staphylococcus aureus
0.156
-
 3'-phosphorylated RNA(n) pH 8.4, 37°C Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus Q7A5X7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-cyclic RNAn + phosphate
-
 Staphylococcus aureus RNA(n-1) + a nucleotide diphosphate
-
 ?
3'-phosphorylated RNA(n) + phosphate
-
 Staphylococcus aureus RNA(n-1) + a nucleotide diphosphate enzyme inefficiently degrades 3'-phosphorylated RNA ?
poly(A)(n-1) + ADP
-
 Staphylococcus aureus poly(A)n + phosphate
-
 r
poly(A)n + phosphate
-
 Staphylococcus aureus poly(A)(n-1) + ADP
-
 r
RNA(n) + phosphate
-
 Staphylococcus aureus RNA(n-1) + a nucleotide diphosphate
-
 ?

General Information

General Information Comment Organism
physiological function the cvfA-deletion mutant phenotype showing decreased agr expression and hemolysin production, is suppressed by disrupting pnpA-encoding PNPase. Loss of the 3'- to 5'-exonuclease activity is required for suppression. CvfA protein hydrolyzes a 2',3'-cyclic phosphodiester bond at the RNA 3' terminus, producing RNA with a 3'-phosphate. Purified PNPase efficiently degrades RNA with 2',3'-cyclic phosphate at the 3' terminus (2',3'-cyclic RNA), but it inefficiently degrades 3'-phosphorylated RNA Staphylococcus aureus