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Literature summary for 2.7.7.8 extracted from

  • Falaleeva, M.V.; Chetverina, H.V.; Ugarov, V.I.; Uzlova, E.A.; Chetverin, A.B.
    Factors influencing RNA degradation by Thermus thermophilus polynucleotide phosphorylase (2008), FEBS J., 275, 2214-2226.
    View publication on PubMed
Search for more literature for 2.7.7.8

Application

Application Comment Organism
synthesis at the optimal temperature, polynucleotide phosphorylase completely destroys RNAs that possess even a very stable intramolecular secondary structure, but leaves intact RNAs whose 3' end is protected by chemical modification or by hybridization with a complementary oligonucleotide. This allows individual RNAs to be isolated from heterogeneous populations by degrading unprotected species. If oligonucleotide is hybridized to an internal RNA segment, the Tth polynucelotide phosphorylase stalls eight nucleotides downstream of that segment. This allows any arbitrary 5'-terminal fragment of RNA to be prepared with a precision similar to that of run-off transcription, but without the need for a restriction site Thermus thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, enzyme retains significant activity when the concentration is in the micromolar range Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q9ZAE1
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information at the optimal temperature, polynucleotide phosphorylase completely destroys RNAs that possess even a very stable intramolecular secondary structure, but leaves intact RNAs whose 3' end is protected by chemical modification or by hybridization with a complementary oligonucleotide. This allows individual RNAs to be isolated from heterogeneous populations by degrading unprotected species. If oligonucleotide is hybridized to an internal RNA segment, the Tth polynucelotide phosphorylase stalls eight nucleotides downstream of that segment. This allows any arbitrary 5'-terminal fragment of RNA to be prepared with a precision similar to that of run-off transcription, but without the need for a restriction site Thermus thermophilus ?
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 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Thermus thermophilus