General Stability | Organism |
---|---|
stable against several freezing and thawing cycles | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 92000 + 1 * 73000 + 1 * 35000, SDS-PAGE | Thermus thermophilus |
73000 | - |
1 * 92000 + 1 * 73000 + 1 * 35000, SDS-PAGE | Thermus thermophilus |
92000 | - |
1 * 92000 + 1 * 73000 + 1 * 35000, SDS-PAGE | Thermus thermophilus |
190000 | - |
native PAGE, gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
HB-8 strain | - |
Purification (Comment) | Organism |
---|---|
ultracentrifugation, ammonium sulfate, QAE-Sephadex a-25, DEAE-cellulose, hydroxyapatite, purified enzyme loses poly(A) phosphorylating activity | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
- |
Thermus thermophilus |
Storage Stability | Organism |
---|---|
-20°C, dilute solution, at least 2 months, no loss of activity | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A) + ADP | primer required for polymerization | Thermus thermophilus | poly(A)+1 + phosphate | - |
r | |
poly(C) + CDP | primer required for polymerization | Thermus thermophilus | poly(C)+1 + phosphate | - |
r | |
poly(G) + GDP | primer required for polymerization | Thermus thermophilus | poly(G)+1 + phosphate | - |
r | |
poly(I) + IDP | primer required for polymerization | Thermus thermophilus | poly(I)+1 + phosphate | - |
r | |
poly(U)+ UDP | primer required for polymerization | Thermus thermophilus | poly(U)+1 + phosphate | - |
r | |
polyadenylic acid + phosphate | - |
Thermus thermophilus | ? + ADP | - |
? | |
polycytidylic acid + phosphate | - |
Thermus thermophilus | ? + CDP | - |
? | |
polyguanylic acid + phosphate | - |
Thermus thermophilus | ? + GDP | - |
? | |
polyuridylic acid + phosphate | - |
Thermus thermophilus | ? + UDP | - |
? | |
RNAn + a nucleoside diphosphate | primer-dependent activity | Thermus thermophilus | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | ADP, GDP, UDP and CDP polymerized to the extent of 7 S size polymer | Thermus thermophilus | RNAn+1 + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 92000 + 1 * 73000 + 1 * 35000, SDS-PAGE | Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
polymerization of UDP with (Ap)3A as primer | Thermus thermophilus |
70 | - |
poymerization of ADP, CDP or GDP with (Ap)3A as primer | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
10 min, 0.05 mg/ml enzyme, less than 10% loss of activity | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.33 | - |
Polyadenylic acid | synthesis of a copolymer of adenylic acid | Thermus thermophilus | |
3.33 | - |
polyguanylic acid | synthesis of a copolymer of guanylic acid | Thermus thermophilus | |
3.33 | - |
polyuridylic acid | synthesis of a copolymer of uridylic acid | Thermus thermophilus | |
3.33 | - |
Polycytidylic acid | synthesis of a copolymer of cytidylic acid | Thermus thermophilus | |
75 | - |
Polyadenylic acid | - |
Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.7 | - |
poymerization of ADP, CDP or UDP | Thermus thermophilus |
10.5 | - |
polymerization of GDP | Thermus thermophilus |