KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000166 | - |
globin mRNA | pH 8.0, 37°C, phosphorolysis of poly(A)tail from rabbit globin mRNA | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
230000 | - |
ultracentrifugation, equilibrium sedimentaion analysis | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-cellulose, RNA-sepharose | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27.67 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 3 mg/ml enzyme concentration, 3 years, no loss of activity | Escherichia coli |
-20°C, dilute solution, at least 2 months, no loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + globin mRNAn | - |
Escherichia coli | globin mRNAn+1 + phosphate | - |
? | |
rabbit globin mRNAn+1 + phosphate | only the poly(A) tail of the mRNA is removed | Escherichia coli | ADP + rabbit globin mRNAn | - |
? | |
RNAn + a nucleoside diphosphate | - |
Escherichia coli | RNAn+1 + phosphate | - |
r | |
RNAn+1 + phosphate | processive phosphorolysis of the poly(A) tail of each globin mRNA chain | Escherichia coli | RNAn + a nucleoside diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
trimer | SDS-PAGE | Escherichia coli |
trimer | alpha3, 3 * 84000-95000, enzyme form A, ultrastructural observations | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Escherichia coli | isoelectric focusing | - |
6.1 |