Any feedback?
Literature summary for 2.7.7.78 extracted from
- A novel GDP-D-glucose phosphorylase involved in quality control of the nucleoside diphosphate sugar pool in Caenorhabditis elegans and mammals (2011), J. Biol. Chem., 286, 21511-21523.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Caenorhabditis elegans |
| expression in Escherichia coli | Homo sapiens |
| expression in Escherichia coli | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
GDP-alpha-D-glucose | pH 7.5, 31°C | Homo sapiens |  |
| 0.01 | - |
GDP-alpha-D-glucose | pH 7.5, 26°C | Caenorhabditis elegans | |
| 1.2 | - |
phosphate | pH 7.5, 26°C | Caenorhabditis elegans | |
| 2.9 | - |
phosphate | pH 7.5, 31°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-alpha-D-glucose + phosphate | Homo sapiens | - |
alpha-D-glucose 1-phosphate + GDP | - |
? | |
| GDP-alpha-D-glucose + phosphate | Mus musculus | - |
alpha-D-glucose 1-phosphate + GDP | - |
? | |
| GDP-alpha-D-glucose + phosphate | Caenorhabditis elegans | the enzyme may be involved in prevention of misincorporation of glucose in place of mannose residues into glycoconjugates i.e. to remove accidentally produced GDP-alpha-D-glucose | alpha-D-glucose 1-phosphate + GDP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | Q5ZR76 | - |
- |
| Homo sapiens | Q6ZNW5 | - |
- |
| Mus musculus | Q3TLS3 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| anterior hypodermis | - |
Caenorhabditis elegans | - |
| brain | highest levels measured in brain and testis (4-8 times higher than in the remaining tissues) | Mus musculus | - |
| embryo | developing | Caenorhabditis elegans | - |
| heart | highest levels measured in brain and testis (4-8 times higher than in the remaining tissues) | Mus musculus | - |
| kidney | highest levels measured in brain and testis (4-8 times higher than in the remaining tissues) | Mus musculus | - |
| larva | expressed in all the normal larval stages observed | Caenorhabditis elegans | - |
| liver | highest levels measured in brain and testis (4-8 times higher than in the remaining tissues) | Mus musculus | - |
| spermatheca | - |
Caenorhabditis elegans | - |
| testis | highest levels measured in brain and testis (4-8 times higher than in the remaining tissues) | Mus musculus | - |
| thymus | highest levels measured in brain and testis (4-8 times higher than in the remaining tissues) | Mus musculus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 10% glycerol | Caenorhabditis elegans |
| -80°C, 10% glycerol | Homo sapiens |
| -80°C, 10% glycerol | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-alpha-D-glucose + phosphate | - |
Homo sapiens | alpha-D-glucose 1-phosphate + GDP | - |
? | |
| GDP-alpha-D-glucose + phosphate | - |
Mus musculus | alpha-D-glucose 1-phosphate + GDP | - |
? | |
| GDP-alpha-D-glucose + phosphate | the enzyme may be involved in prevention of misincorporation of glucose in place of mannose residues into glycoconjugates i.e. to remove accidentally produced GDP-alpha-D-glucose | Caenorhabditis elegans | alpha-D-glucose 1-phosphate + GDP | - |
? | |
| GDP-alpha-D-glucose + phosphate | activities with GDP-L-galactose, GDP-D-mannose and UDP-D-glucose are all less than 3% that with GDP-D-glucose | Caenorhabditis elegans | alpha-D-glucose 1-phosphate + GDP | - |
? | |
| GDP-alpha-D-glucose + phosphate | activities with GDP-L-galactose, GDP-D-mannose and UDP-D-glucose are all less than 3% that with GDP-D-glucose | Homo sapiens | alpha-D-glucose 1-phosphate + GDP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C10F3.4 | - |
Caenorhabditis elegans |
| C15orf58 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 26 | - |
assay at | Caenorhabditis elegans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26 | - |
GDP-alpha-D-glucose | pH 7.5, 31°C | Homo sapiens | |
| 28.1 | - |
phosphate | pH 7.5, 31°C | Homo sapiens | |
| 265 | - |
GDP-alpha-D-glucose | pH 7.5, 26°C | Caenorhabditis elegans | |
| 380 | - |
phosphate | pH 7.5, 26°C | Caenorhabditis elegans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Caenorhabditis elegans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.7 | - |
phosphate | pH 7.5, 31°C | Homo sapiens | |
| 317 | - |
phosphate | pH 7.5, 26°C | Caenorhabditis elegans | |
| 1300 | - |
GDP-alpha-D-glucose | pH 7.5, 31°C | Homo sapiens | |
| 26500 | - |
GDP-alpha-D-glucose | pH 7.5, 26°C | Caenorhabditis elegans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
