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Literature summary for 2.7.7.72 extracted from

  • Czech, A.
    Deep sequencing of tRNAs 3-termini sheds light on CCA-tail integrity and maturation (2020), RNA, 26, 199-208 .
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
analysis development of a tRNA sequencing method that is specifically tailored to assess the 3'-termini of Escherichia coli tRNA Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Escherichia coli CA244
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General Information

General Information Comment Organism
metabolism under exponential growth conditions, a significant fraction of tRNAs with damaged CCA-tails is found and this fraction decreases upon transition into stationary phase. tRNAs bearing guanine as a discriminator base are generally unaffected by CCA-tail damage. The knockout of the repairing CCA-adding enzyme significantly reduces tRNA integrity, and tRNACys integrity is reduced from 82% in wild-type to 40% in the knockout strain. Even slight reduction of CCA integrity in exponential phase tRNA results in reduced protein synthesis Escherichia coli