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Literature summary for 2.7.7.72 extracted from

  • Erber, L.; Hoffmann, A.; Fallmann, J.; Hagedorn, M.; Hammann, C.; Stadler, P.; Betat, H.; Prohaska, S.; Moerl, M.
    Unusual occurrence of two bona-fide cca-adding enzymes in Dictyostelium discoideum (2020), Int. J. Mol. Sci., 21, 1-16 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.72

Cloned(Commentary)

Cloned (Comment) Organism
codon-optimized expression in Escherichia coli Dictyostelium discoideum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol even distribution in the cytosol Dictyostelium discoideum 5829
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mitochondrion both isoforms Cca1 and CCa2 are not specifically enriched in mitochondria Dictyostelium discoideum 5739
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Organism

Organism UniProt Comment Textmining
Dictyostelium discoideum Q54BQ2 isoform Cca2
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Dictyostelium discoideum Q55BE1 isoform Cca1
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information both isoforms Cca1 and Cca2 accept the complete sets of cytosolic and mitochondrial tRNAs at the individual developmental stages. Isoform Cca2 shows efficient binding to the substrates with a Kd of 2.3 microM and 1.7 microM for the in vitro transcript and the in vivo tRNA preparation, respectively. Isoform Cca1 shows almost no binding at any protein concentration Dictyostelium discoideum ?
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tRNA(Leu) precursor + 2 CTP + ATP
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 Dictyostelium discoideum tRNA(Leu) with a 3'CCA end + 3 diphosphate
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 ?
tRNA(Ser) precursor + 2 CTP + ATP
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 Dictyostelium discoideum tRNA(Ser) with a 3'CCA end + 3 diphosphate
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 ?

Synonyms

Synonyms Comment Organism
CCA1
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 Dictyostelium discoideum
DDB0191978
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 Dictyostelium discoideum
DDB0216798
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 Dictyostelium discoideum
polyA_pol domain-containing protein
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 Dictyostelium discoideum

Expression

Organism Comment Expression
Dictyostelium discoideum expression of isoforms Cca1 and Cca2 is cell-cycle-dependent and inversely regulated. In the unicellular state, Cca1 is highly expressed. After the onset of aggregation, transcription of cca1 is downregulated and raises again when the cells switch from aggregation to mound formation until the final fruiting body is formed. Cca2 exhibits a very low expression in amoeba, is upregulated as the developmental process into the aggregation is started and declines for the rest of the development additional information