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Literature summary for 2.7.7.72 extracted from

  • Kuhn, C.D.; Wilusz, J.E.; Zheng, Y.; Beal, P.A.; Joshua-Tor, L.
    On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme (2015), Cell, 160, 644-658.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
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 Archaeoglobus fulgidus

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with a human MenBeta minihelix. The unstable minihelix is bound between the enzyme’s catalytic center, comprised of the head and neck domains, and its tail domain. The minihelix perfectly mimics full-length tRNA with its acceptor and TPsiC stems folding into a continuous A-type RNA helix. The TPsiC loop is in the same conformation as in full-length tRNA Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O28126
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information tRNA minihelices, which contain only the acceptor stem and TPsiC stem-loop, are also efficiently subjected to CCACCA addition when they have guanosines at the first and second positions as well as a destabilized acceptor stem by virtue of mismatches and G-U wobbles Archaeoglobus fulgidus ?
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General Information

General Information Comment Organism
physiological function whereas CCA is added to stable tRNAs and tRNA-like transcripts, a second CCA repeat is added to certain unstable transcripts to initiate their degradation. Following the first CCA addition cycle, nucleotide binding to the active site triggers a clockwise screw motion, producing torque on the RNA. This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle. Intriguingly, with the CCA-adding enzyme acting as a molecular vise, the RNAs proofread themselves through differential responses to its interrogation between stable and unstable substrates Archaeoglobus fulgidus