Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of variants bearing modifications within the body domain and bearing alterations at the extreme C-terminus of the tail domain. Mutations do not alter the overall secondary structure of the proteins to a large extent. Like wild-type, the mutant with alterations at the extreme C-terminus of the tail domain also adds a complete CCA sequence, while the mutant with modifications within the body domain and a double mutant show a complete loss of activity. A variant lacking the C-terminal nine amino acids shows a reduced rate of CCA addition in vitro when compared with the native enzyme or the mutant with alterations at the extreme C-terminus. The first five amino acids of the N-terminal signal sequence are required for efficient translocation into mitochondria. Variants lacking the first start codon show less mitochondrial import. Mutations in the mature domain reduce the localization tomitochondria and in turn enhance the translocation into chloroplasts | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
a tRNA precursor | wild-type, pH 9.0, 37°C | Arabidopsis thaliana | |
0.012 | - |
a tRNA precursor | the mutant with alterations at the extreme C-terminus of the tail domain, pH 9.0, 37°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | the first five amino acids of the N-terminal signal sequence are required for efficient translocation into mitochondria. Variants lacking the first start codon show less mitochondrial import. Mutations in the mature domain reduce the localization tomitochondria and in turn enhance the translocation into chloroplasts | Arabidopsis thaliana | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a tRNA precursor + 2 CTP + ATP | - |
Arabidopsis thaliana | a tRNA with a 3'CCA end + 3 diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tRNA-NT | - |
Arabidopsis thaliana |