Protein Variants | Comment | Organism |
---|---|---|
D106A | CTP-adding activity is 90% of wild-type level, ATP-adding activities is 14% of wild-type level | Saccharolobus shibatae |
D106A | mutation has no effect on CCA-adding activity | Saccharolobus shibatae |
D124A | mutation has no effect on CCA-adding activity | Saccharolobus shibatae |
D143A | mutation has no effect on CCA-adding activity | Saccharolobus shibatae |
D215A | CTP-adding activity is 16% of wild-type level, ATP-adding activities is 13% of wild-type level | Saccharolobus shibatae |
D215A | mutation has no effect on CCA-adding activity | Saccharolobus shibatae |
D218A | mutation has no effect on CCA-adding activity | Saccharolobus shibatae |
D53A | no activity | Saccharolobus shibatae |
D53A/D55A | no activity | Saccharolobus shibatae |
D55A | no activity | Saccharolobus shibatae |
D55E | no activity | Saccharolobus shibatae |
delC135 | no activity | Saccharolobus shibatae |
delC30 | no activity | Saccharolobus shibatae |
E104A | mutation has no effect on CCA-adding activity | Saccharolobus shibatae |
E114A | mutation has no effect on CCA-adding activity | Saccharolobus shibatae |
E139A | mutation has no effect on CCA-adding activity | Saccharolobus shibatae |
E144A | mutation has no effect on CCA-adding activity | Saccharolobus shibatae |
E161A | mutation has no effect on CCA-adding activity | Saccharolobus shibatae |
E173A | CTP-adding activity is 19% of wild-type level, ATP-adding activities is 7% of wild-type level | Saccharolobus shibatae |
E173A | mutation has no effect on CCA-adding activity | Saccharolobus shibatae |
E209A | mutation has no effect on CCA-adding activity | Saccharolobus shibatae |
G156D | neither CTP- nor ATP-adding activity is significantly affected | Saccharolobus shibatae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus shibatae | P77978 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a tRNA precursor + 2 CTP + ATP | one catalytic center is responsible for addition of both CTP and ATP. As the single active site catalyzes addition of each nucleotide, the growing 3'-end of the tRNA would progressively refold to create a binding pocket for addition of the next nucleotide | Saccharolobus shibatae | a tRNA with a 3' CCA end + 3 diphosphate | - |
? | |
a tRNA precursor + CTP | one catalytic center is responsible for addition of both CTP and ATP. As the single active site catalyzes addition of each nucleotide, the growing 3'-end of the tRNA would progressively refold to create a binding pocket for addition of the next nucleotide | Saccharolobus shibatae | a tRNA with a 3' cytidine end + diphosphate | - |
? | |
a tRNA with a 3' CC end + ATP | one catalytic center is responsible for addition of both CTP and ATP. As the single active site catalyzes addition of each nucleotide, the growing 3'-end of the tRNA would progressively refold to create a binding pocket for addition of the next nucleotide | Saccharolobus shibatae | a tRNA with a 3' CCA end + diphosphate | - |
? | |
a tRNA with a 3' cytidine + CTP | one catalytic center is responsible for addition of both CTP and ATP. As the single active site catalyzes addition of each nucleotide, the growing 3'-end of the tRNA would progressively refold to create a binding pocket for addition of the next nucleotide | Saccharolobus shibatae | a tRNA with a 3' CC end + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CCA-adding enzyme | - |
Saccharolobus shibatae |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme synthesizes and repairs the 3'-terminal CCA sequence of tRNA | Saccharolobus shibatae |