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Literature summary for 2.7.7.72 extracted from

  • Seth, M.; Thurlow, D.L.; Hou, Y.M.
    Poly(C) synthesis by class I and class II CCA-adding enzymes (2002), Biochemistry, 41, 4521-4532.
    View publication on PubMed
Search for more literature for 2.7.7.72

Protein Variants

Protein Variants Comment Organism
A75C as compared to wild-type enzyme the Km increases 2.7-fold from A addition to C addition, while the kcat decreased 14fold. The overall kcat/Km ratio of C addition to A addition is 0.03 Saccharolobus shibatae
V34C as compared to wild-type enzyme the Km increases 1.4fold from A addition to C addition, while the kcat decreases 18fold. The overall kcat/Km ratio of C addition to A addition is 0.04 Saccharolobus shibatae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
 ATP pH 8.1, 70°C, mutant enzyme A75C Saccharolobus shibatae
0.145
-
 CTP pH 8.1, 70°C, mutant enzyme A75C Saccharolobus shibatae
0.263
-
 ATP pH 8.1, 70°C, mutant enzyme V34C Saccharolobus shibatae
0.366
-
 CTP pH 8.1, 70°C, mutant enzyme V34C Saccharolobus shibatae
0.5
-
 ATP pH 8.1, 70°C, wild-type enzyme Saccharolobus shibatae
0.515
-
 CTP pH 8.1, 70°C, wild-type enzyme Saccharolobus shibatae

Organism

Organism UniProt Comment Textmining
Saccharolobus shibatae P77978
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a tRNA(Ala) precursor + 2 CTP + ATP synthesizes poly(C) when incubated with CTP alone, but switches to synthesize CCA when incubated with both CTP and ATP. The enzyme also exhibits a processing activity that removes nucleotides in the 3' to 5' direction to as far as position 74 Saccharolobus shibatae a tRNA(Ala) with a 3' CCA end + 3 diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
ATP(CTP):tRNA nucleotidyl transferase
-
 Saccharolobus shibatae
CCA-adding enzyme
-
 Saccharolobus shibatae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Saccharolobus shibatae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0012
-
 CTP pH 8.1, 70°C, mutant enzyme V34C Saccharolobus shibatae
0.0013
-
 CTP pH 8.1, 70°C, mutant enzyme A75C Saccharolobus shibatae
0.0013
-
 ATP pH 8.1, 70°C, mutant enzyme V34C Saccharolobus shibatae
0.0027
-
 CTP pH 8.1, 70°C, wild-type enzyme Saccharolobus shibatae
0.0183
-
 ATP pH 8.1, 70°C, mutant enzyme A75C Saccharolobus shibatae
0.035
-
 ATP pH 8.1, 70°C, wild-type enzyme Saccharolobus shibatae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0033
-
 CTP pH 8.1, 70°C, mutant enzyme V34C Saccharolobus shibatae
0.005
-
 ATP pH 8.1, 70°C, mutant enzyme V34C Saccharolobus shibatae
0.005
-
 CTP pH 8.1, 70°C, wild-type enzyme Saccharolobus shibatae
0.09
-
 CTP pH 8.1, 70°C, mutant enzyme A75C Saccharolobus shibatae
0.34
-
 ATP pH 8.1, 70°C, mutant enzyme A75C Saccharolobus shibatae
0.7
-
 ATP pH 8.1, 70°C, wild-type enzyme Saccharolobus shibatae