Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
2 * 48000, the 48 kDa monomer forms a stable salt-resistant dimer in solution. Further dimerization of the dimeric enzyme to form a tetramer is induced by the binding of two tRNA molecules | Saccharolobus shibatae |
98000 | - |
dimer, gel filtration | Saccharolobus shibatae |
242000 | - |
tetrameric enzyme with two bound tRNA(Phe)s, gel filtration | Saccharolobus shibatae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus shibatae | P77978 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA(Phe) precursor + 2 CTP + ATP | the 48 kDa monomer forms a stable salt-resistant dimer in solution. Further dimerization of the dimeric enzyme to form a tetramer is induced by the binding of two tRNA molecules. The formation of a tetramer with only two bound tRNA molecules leads to the suggestion that one pair of active sites may be specific for adding two C bases, which results in scrunching of the primer strand. An adjacent second pair of active sites may be specific for adding A after addition of two C bases which makes the 30 terminus long enough to reach the second pair of active sites | Saccharolobus shibatae | tRNA(Phe) with a 3' CCA end + 3 diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 48000, the 48 kDa monomer forms a stable salt-resistant dimer in solution. Further dimerization of the dimeric enzyme to form a tetramer is induced by the binding of two tRNA molecules | Saccharolobus shibatae |
tetramer | 2 * 48000, the 48 kDa monomer forms a stable salt-resistant dimer in solution. Further dimerization of the dimeric enzyme to form a tetramer is induced by the binding of two tRNA molecules | Saccharolobus shibatae |
Synonyms | Comment | Organism |
---|---|---|
CCA-adding enzyme | - |
Saccharolobus shibatae |