Cloned (Comment) | Organism |
---|---|
mitochondrial CCase, expression as N-terminally His-tagged enzyme in Escherichia coli strain BL21 CodonPlus (DE3)-RP | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant monomeric native enzyme or enzyme in complex with thimerosal, sitting drop vapor diffusion method, mixing of 0.003-0.005 ml of protein solution with 0.001 ml of reservoir solution containing 100 mM sodium citrate, pH 5.6, 2.2 M ammonium sulfate, and equilibration against 0.4 ml of reservoir solution, 3 days to 3 weeks, for ligand bound enzyme crystals soaking in reservoir solution containing 0.5 mM thimerosal for 45 min, 18°C, X-ray diffraction structure determination and analysis at 3.4-3.7 A resolution, single isomorphous replacement | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | human mitochondrial tRNA-nucleotidyltransferase | Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme catalyses a unique template-independent but sequence-specific nucleotide polymerization reaction, active site structure and molecular mechanism, overview. Construction of a corkscrew model for CCA addition that includes a fixed active site and a traveling tRNA-binding region formed by flexible parts of the protein | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96Q11 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged mitochondrial CCase from Escherichia coli strain BL21 CodonPlus (DE3)-RP by nickel affinity chromatography and gel filtration to over 95% purity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyses a unique template-independent but sequence-specific nucleotide polymerization reaction, active site structure and molecular mechanism, overview. Construction of a corkscrew model for CCA addition that includes a fixed active site and a traveling tRNA-binding region formed by flexible parts of the protein | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | four domain architecture with a cluster of conserved residues forming a positively charged cleft between the first two domains, modeling of human mitochondrial tRNA-nucleotidyltransferase monomer | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ATP(CTP):tRNA nucleotidyltransferase | - |
Homo sapiens |
CCA adding enzyme | - |
- |
CCA-adding enzyme | - |
Homo sapiens |
CCase | - |
Homo sapiens |
hmtCCase | - |
- |
General Information | Comment | Organism |
---|---|---|
physiological function | all tRNA molecules carry the invariant sequence CCA at their 3'-terminus for amino acid attachment. The post-transcriptional addition of CCA is carried out by ATP(CTP):tRNA nucleotidyltransferase, also called CCase. This enzyme catalyses a unique template-independent but sequence-specific nucleotide polymerization reaction | Homo sapiens |