Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.72 extracted from

  • Augustin, M.A.; Reichert, A.S.; Betat, H.; Huber, R.; Morl, M.; Steegborn, C.
    Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization (2003), J. Mol. Biol., 328, 985-994.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mitochondrial CCase, expression as N-terminally His-tagged enzyme in Escherichia coli strain BL21 CodonPlus (DE3)-RP Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant monomeric native enzyme or enzyme in complex with thimerosal, sitting drop vapor diffusion method, mixing of 0.003-0.005 ml of protein solution with 0.001 ml of reservoir solution containing 100 mM sodium citrate, pH 5.6, 2.2 M ammonium sulfate, and equilibration against 0.4 ml of reservoir solution, 3 days to 3 weeks, for ligand bound enzyme crystals soaking in reservoir solution containing 0.5 mM thimerosal for 45 min, 18°C, X-ray diffraction structure determination and analysis at 3.4-3.7 A resolution, single isomorphous replacement Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion human mitochondrial tRNA-nucleotidyltransferase Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme catalyses a unique template-independent but sequence-specific nucleotide polymerization reaction, active site structure and molecular mechanism, overview. Construction of a corkscrew model for CCA addition that includes a fixed active site and a traveling tRNA-binding region formed by flexible parts of the protein ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96Q11
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged mitochondrial CCase from Escherichia coli strain BL21 CodonPlus (DE3)-RP by nickel affinity chromatography and gel filtration to over 95% purity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme catalyses a unique template-independent but sequence-specific nucleotide polymerization reaction, active site structure and molecular mechanism, overview. Construction of a corkscrew model for CCA addition that includes a fixed active site and a traveling tRNA-binding region formed by flexible parts of the protein Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
More four domain architecture with a cluster of conserved residues forming a positively charged cleft between the first two domains, modeling of human mitochondrial tRNA-nucleotidyltransferase monomer Homo sapiens

Synonyms

Synonyms Comment Organism
ATP(CTP):tRNA nucleotidyltransferase
-
Homo sapiens
CCA adding enzyme
-
-
CCA-adding enzyme
-
Homo sapiens
CCase
-
Homo sapiens
hmtCCase
-
-

General Information

General Information Comment Organism
physiological function all tRNA molecules carry the invariant sequence CCA at their 3'-terminus for amino acid attachment. The post-transcriptional addition of CCA is carried out by ATP(CTP):tRNA nucleotidyltransferase, also called CCase. This enzyme catalyses a unique template-independent but sequence-specific nucleotide polymerization reaction Homo sapiens