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Literature summary for 2.7.7.72 extracted from
- Purification and some properties of Escherichia coli tRNA nucleotidyltransferase (1977), J. Biol. Chem., 252, 5584-5588.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | inhibition of AMP incorporating activity only, the CMP incorporating activity is much less sensitive | Escherichia coli |  |
| 1,7-phenanthroline | slight inhibition | Escherichia coli | |
| 2,2,2-Terpyridyl | inhibition of AMP incorporating activity only, the CMP incorporating activity is much less sensitive | Escherichia coli | |
| 2,2-dipyridyl | at high concentrations, inhibition of AMP incorporating activity only, the CMP incorporating activity is much less sensitive | Escherichia coli | |
| bathophenanthroline | inhibition of AMP incorporating activity only, the CMP incorporating activity is much less sensitive | Escherichia coli | |
| additional information | no inhibition by EDTA | Escherichia coli | |
| Neocuproine | slight inhibition | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51500 | - |
1 * 51500, SDS-PAGE | Escherichia coli |
| 53000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 11800fold by ammonium sulfate fractionation, anion exchange and hydroxylapatite chromatography, and tRNA affinity chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 67.1 | - |
purified enzyme, pH not specified in the publication, 37°C, pooled column fraction | Escherichia coli |
| 280 | - |
purified enzyme, pH not specified in the publication, 37°C, column peak fraction | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tRNAX1 + ATP + 2 CTP | only one protein is responsible for both AMP and CMP incorporation | Escherichia coli | tRNAXCCA + 3 diphosphate | - |
r | |
| tRNAX1 + ATP + 2 CTP | only one protein is responsible for both AMP and CMP incorporation | Escherichia coli B / ATCC 11303 | tRNAXCCA + 3 diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 51500, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| adenylyl (cytidylyl)-tRNA nucleotidyltransferase | - |
Escherichia coli |
| ATP(CTP): tRNA adenylyl (cytidylyl)transferase | - |
Escherichia coli |
| tRNA nucleotidyltransferase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | both AMP- and CMP-incorporating activities, isoelectric focusing | - |
5.85 |
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