Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Smith, M.R.; Alnajjar, K.S.; Hoitsma, N.M.; Sweasy, J.B.; Freudenthal, B.D.
    Molecular and structural characterization of oxidized ribonucleotide insertion into DNA by human DNA polymerase beta (2020), J. Biol. Chem., 295, 1613-1622 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
the Y271G mutation is overexpressed in BL21-CodonPlus (DE3)-RP Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion, ternary polbeta:DNA:r8-oxo-GTP crystals Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y271G the mutant is rationally designed to provide flexibility to the steric gate backbone carboxyl of Tyr-271 in pol beta Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P06746
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-oxodGTP + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?
dGTP + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?
r8-oxo-GTP + DNAn relative to correct dGTP insertion, r8-oxo-GTP insertion efficiency opposite dC and dA is reduced more than 250000fold and 4000fold, respectively. Insertion of r8-oxo-GTP is less efficient than 8-oxodGTP by 700- and 4300fold opposite dA and dC, respectively Homo sapiens diphosphate + DNAn+1
-
 ?
rGTP + DNAn
-
 Homo sapiens diphosphate + DNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
DNA polymerase beta
-
 Homo sapiens