Literature summary for 2.7.7.7 extracted from

Hamal, A.; Forterre, P.; Elie, C.
Purification and characterization of a DNA polymerase from the archaebacterium Thermoplasma acidophilum (1990), Eur. J. Biochem., 190, 517-521 .

Search for more literature for 2.7.7.7

Activating Compound

Activating Compound	Comment	Organism	Structure
ethyleneglycol	5%, activity is stimulated by 30%	Thermoplasma acidophilum
glycerol	5%, activity is stimulated by 30%	Thermoplasma acidophilum
additional information	no stimulation with poly(ethy1ene glycol)	Thermoplasma acidophilum

Inhibitors

Inhibitors	Comment	Organism	Structure
2',3'-dideoxyribosylthymine triphosphate	the enzyme is slightly sensitive to. 25% inhibition is observed with a ddTTP/dTTP ratio of 50	Thermoplasma acidophilum
additional information	the enzyme is resistant to aphidicolin	Thermoplasma acidophilum
N-ethylmaleimide	inhibited when preincubation with this reagent is performed at 65°C	Thermoplasma acidophilum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
MgCl2	optimal activity is obtained in the presence of 4 mM MgCl2	Thermoplasma acidophilum
MnCl2	sustitution of MgCl2 by MnCl2 produces a slight decrease of activity. Optimal activity is obtained in the presence of 0.4 mM MgCl2	Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
88000	-	gel filtration	Thermoplasma acidophilum

Organism

Organism	UniProt	Comment	Textmining
Thermoplasma acidophilum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	a 3'->5' exonuclease activity is associated with the purified DNA polymerase	Thermoplasma acidophilum	?	-	?
poly(rA)/(dT)12 + dTTP	-	Thermoplasma acidophilum	poly(rA)/(dT)13 + diphosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 88000, SDS-PAGE	Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	DNA polymerase activity and 3'->5' exonuclease activity	Thermoplasma acidophilum

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
50	-	55% of maximal activity, DNA polymerase activity	Thermoplasma acidophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
65	-	stable up to	Thermoplasma acidophilum
75	-	30 min, 50% loss of activity	Thermoplasma acidophilum
85	-	30 min, vomplete inactivation	Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Thermoplasma acidophilum

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Release 2023.1 (January 2023)