Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Seo, K.J.; Cho, S.S.; Ppyun, H.W.; Youn, M.H.; Kim, S.H.; Seo, B.S.; Kwon, S.T.
    Characterization of a family B DNA polymerase from the hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I and its application to PCR (2014), Appl. Biochem. Biotechnol., 173, 108-120.
    View publication on PubMed

Application

Application Comment Organism
molecular biology optimal conditions for polymerase chain reaction are determined. Iho DNA polymerase possesses 3'->5' exonuclease activity, and the fidelity of the Iho DNA polymerase is similar to that of Pfu and Vent DNA polymerases. However, Iho DNA polymerase provides more enhanced efficiency of PCR amplification than Pfu and Vent DNA polymerases. Iho DNA polymerase can successfully amplify a 2-kb lambda DNA target with a 10/s extension time and could amplify a DNA fragment up to 8 kb lambda DNA Ignicoccus hospitalis

Cloned(Commentary)

Cloned (Comment) Organism
highly expressed under the control of T7lac promoter of pET-28ARG in Escherichia coli BL21-CodonPlus(DE3)-RIL cells Ignicoccus hospitalis

Metals/Ions

Metals/Ions Comment Organism Structure
KCl optimal concentration of KCl is 60 mM Ignicoccus hospitalis
Mg2+ strongly activated by the presence of Mg2+ at an optimum concentration of 3 mM Ignicoccus hospitalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
x * 88000, SDS-PAGE Ignicoccus hospitalis

Organism

Organism UniProt Comment Textmining
Ignicoccus hospitalis A8A8J4
-
-
Ignicoccus hospitalis DSM 18386 A8A8J4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Ignicoccus hospitalis

Subunits

Subunits Comment Organism
? x * 88000, SDS-PAGE Ignicoccus hospitalis

Synonyms

Synonyms Comment Organism
Igni_0062 locus name Ignicoccus hospitalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Ignicoccus hospitalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 80 65°C: about 60% of maximal activity, 80°C: about 60% of maximal activity Ignicoccus hospitalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
94
-
half-life is about 2 h Ignicoccus hospitalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Ignicoccus hospitalis

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 30% of maximal activity, pH 9.0: about 75% of maximal activity Ignicoccus hospitalis