Application | Comment | Organism |
---|---|---|
molecular biology | optimal conditions for polymerase chain reaction are determined. Iho DNA polymerase possesses 3'->5' exonuclease activity, and the fidelity of the Iho DNA polymerase is similar to that of Pfu and Vent DNA polymerases. However, Iho DNA polymerase provides more enhanced efficiency of PCR amplification than Pfu and Vent DNA polymerases. Iho DNA polymerase can successfully amplify a 2-kb lambda DNA target with a 10/s extension time and could amplify a DNA fragment up to 8 kb lambda DNA | Ignicoccus hospitalis |
Cloned (Comment) | Organism |
---|---|
highly expressed under the control of T7lac promoter of pET-28ARG in Escherichia coli BL21-CodonPlus(DE3)-RIL cells | Ignicoccus hospitalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimal concentration of KCl is 60 mM | Ignicoccus hospitalis | |
Mg2+ | strongly activated by the presence of Mg2+ at an optimum concentration of 3 mM | Ignicoccus hospitalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
x * 88000, SDS-PAGE | Ignicoccus hospitalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ignicoccus hospitalis | A8A8J4 | - |
- |
Ignicoccus hospitalis DSM 18386 | A8A8J4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Ignicoccus hospitalis |
Subunits | Comment | Organism |
---|---|---|
? | x * 88000, SDS-PAGE | Ignicoccus hospitalis |
Synonyms | Comment | Organism |
---|---|---|
Igni_0062 | locus name | Ignicoccus hospitalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Ignicoccus hospitalis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 80 | 65°C: about 60% of maximal activity, 80°C: about 60% of maximal activity | Ignicoccus hospitalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
94 | - |
half-life is about 2 h | Ignicoccus hospitalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Ignicoccus hospitalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 30% of maximal activity, pH 9.0: about 75% of maximal activity | Ignicoccus hospitalis |