Crystallization (Comment) | Organism |
---|---|
the structure of the enzyme bound to G*T-mispaired primer template in the presence of an incoming nucleotide is solved. As a control the structure of the enzyme bound to a matched A-T base pair at the primer terminus is also determined. The structures offer a basis for the low efficiency of the enzyme in extending a G*T mispair: a reverse wobble that deflects the primer 3'-OH away from the incoming nucleotide | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pH 7.5, 24°C, steady-state kinetic parameters for mispair extension by Dpo4 | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97W02 | - |
- |
Saccharolobus solfataricus P2 | Q97W02 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | the enzyme is inefficient at extending mispairs opposite a template G or T, which include, a G*T mispair expected to conform closely to Watson-Crick geometry. It is hindered in extending a G*T mismatch by a reverse wobble | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme is inefficient at extending mispairs opposite a template G or T, which include, a G*T mispair expected to conform closely to Watson-Crick geometry. It is hindered in extending a G*T mismatch by a reverse wobble | Saccharolobus solfataricus P2 | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dpo4 | - |
Saccharolobus solfataricus |
SSO2448 | locus name | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pH 7.5, 24°C, steady-state kinetic parameters for mispair extension by Dpo4 | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pH 7.5, 24°C, steady-state kinetic parameters for mispair extension by Dpo4 | Saccharolobus solfataricus |