Literature summary for 2.7.7.7 extracted from

Stover, J.S.; Chowdhury, G.; Zang, H.; Guengerich, F.P.; Rizzo, C.J.
Translesion synthesis past the C8- and N2-deoxyguanosine adducts of the dietary mutagen 2-amino-3-methylimidazo[4,5-f]quinoline in the NarI recognition sequence by prokaryotic DNA polymerases (2006), Chem. Res. Toxicol., 19, 1506-1517.

Search for more literature for 2.7.7.7

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetic parameters for single-nucleotide incorporation opposite the C8- and N2-2-amino-3-methylimidazo[4,5-f]quinoline adducts of dGuo at the G3- and G1-positions of the NarI recognition sequence by Dpo4	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q97W02	-	-
Saccharolobus solfataricus P2	Q97W02	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
deoxynucleoside triphosphate + DNAn	incorporated of 2-amino-3-methylimidazo[4,5-f]quinoline C8- and N2-dGuo adducts into the G1- and G3-positions of the NarI recognition sequence (5'-G1G2CG3CC-3'), which is a hotspot for arylamine modification. Replication of the C8-adduct at the G3-position results in two-base deletion, whereas error-free bypass and extension is observed at the G1-position. The N2-adduct is bypassed and extended when positioned at the G1-position, and the error-free product is observed. The N2-adduct at the G3-position is more blocking and is bypassed and extended only by Dpo4 to produce an errorfree product. The replication of the 2-amino-3-methylimidazo[4,5-f]quinoline-adducts of dGuo is strongly influenced by the local sequence and the regioisomer of the adduct	Saccharolobus solfataricus	diphosphate + DNAn+1	-	?
deoxynucleoside triphosphate + DNAn	incorporated of 2-amino-3-methylimidazo[4,5-f]quinoline C8- and N2-dGuo adducts into the G1- and G3-positions of the NarI recognition sequence (5'-G1G2CG3CC-3'), which is a hotspot for arylamine modification. Replication of the C8-adduct at the G3-position results in two-base deletion, whereas error-free bypass and extension is observed at the G1-position. The N2-adduct is bypassed and extended when positioned at the G1-position, and the error-free product is observed. The N2-adduct at the G3-position is more blocking and is bypassed and extended only by Dpo4 to produce an errorfree product. The replication of the 2-amino-3-methylimidazo[4,5-f]quinoline-adducts of dGuo is strongly influenced by the local sequence and the regioisomer of the adduct	Saccharolobus solfataricus P2	diphosphate + DNAn+1	-	?

Synonyms

Synonyms	Comment	Organism
Dpo4	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetic parameters for single-nucleotide incorporation opposite the C8- and N2-2-amino-3-methylimidazo[4,5-f]quinoline adducts of dGuo at the G3- and G1-positions of the NarI recognition sequence by Dpo4	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	assay at	Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetic parameters for single-nucleotide incorporation opposite the C8- and N2-2-amino-3-methylimidazo[4,5-f]quinoline adducts of dGuo at the G3- and G1-positions of the NarI recognition sequence by Dpo4	Saccharolobus solfataricus

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Release 2023.1 (January 2023)