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Literature summary for 2.7.7.7 extracted from

  • Uemori, T.; Sato, Y.; Kato, I.; Doi, H.; Ishino, Y.
    A novel DNA polymerase in the hyperthermophilic archaeon, Pyrococcus furiosus: gene cloning, expression, and characterization (1997), Genes Cells, 2, 499-512.
    View publication on PubMed
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
additional information DNA polymerizing activity is not inhibited by aphidicolin at concentrations up to 2 mM that inhibit the activities of Pfu Pol I and other alpha-like DNA polymerases. Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69294
-
 x * 69294 + x + 143161, calculated from sequence Pyrococcus furiosus
218000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Pyrococcus furiosus the enzyme has a template-primer preference which is characteristic of a replicative DNA polymerase diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus P81412 and P81409 P81412: DP1, small subunit. P81409: DP2, large subunit
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn the enzyme has a template-primer preference which is characteristic of a replicative DNA polymerase Pyrococcus furiosus diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn replicative DNA polymerase Pyrococcus furiosus diphosphate + DNAn+1
-
 ?
additional information the enzyme has strong 3'->5' exonucleolytic activity and has a template-primer preference which is characteristic of a replicative DNA polymerase Pyrococcus furiosus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 69294 + x + 143161, calculated from sequence Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 more than 80% of the initial activity is retained after incubation for 30 min Pyrococcus furiosus

General Information

General Information Comment Organism
physiological function replicative DNA polymerase Pyrococcus furiosus