Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pyrococcus abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | - |
- |
- |
Pyrococcus abyssi Orsay | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | PCR performance and fidelity parameters are highest in the presence of 20 mM Tris-HCl, pH 9.0, 1.5 mM MgSO4, 25 mM KCl, 10 mM (NH4)2SO4 and 40 microM of each dNTP. Under these conditions, the error rate is 0.66.10(-6) mutations/nucleotide/duplication | Pyrococcus abyssi | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | PCR performance and fidelity parameters are highest in the presence of 20 mM Tris-HCl, pH 9.0, 1.5 mM MgSO4, 25 mM KCl, 10 mM (NH4)2SO4 and 40 microM of each dNTP. Under these conditions, the error rate is 0.66.10(-6) mutations/nucleotide/duplication | Pyrococcus abyssi Orsay | diphosphate + DNAn+1 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
half-life: 5 h | Pyrococcus abyssi |