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Literature summary for 2.7.7.7 extracted from
- Characterization of two DNA polymerases from the hyperthermophilic euryarchaeon Pyrococcus abyssi (2001), Eur. J. Biochem., 268, 5961-5969.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pyrococcus abyssi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aphidicolin | - |
Pyrococcus abyssi |  |
| additional information | no inhibition by 0.1 mM aphidicolin | Pyrococcus abyssi |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | optimal concentration: 40 mM | Pyrococcus abyssi | |
| KCl | optimal concentration: 50-80 mM | Pyrococcus abyssi | |
| Mg2+ | optimal concentration: 3 mM | Pyrococcus abyssi | |
| Mg2+ | oprtimal concentration: 15-20 mM | Pyrococcus abyssi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68100 | - |
x * 68100 (polB) + x * 139700 (polc), calculated from sequence | Pyrococcus abyssi |
| 90000 | - |
x * 90000, SDS-PAGE | Pyrococcus abyssi |
| 90000 | - |
x * 90000 (polB) + x * 140000 (polC), SDS-PAGE | Pyrococcus abyssi |
| 139700 | - |
x * 68100 (polB) + x * 139700 (polc), calculated from sequence | Pyrococcus abyssi |
| 140000 | - |
x * 90000 (polB) + x * 140000 (polC), SDS-PAGE | Pyrococcus abyssi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus abyssi | P0CL76 | - |
- |
| Pyrococcus abyssi | Q9V2F3 and Q9V2F4 | small subunit polB: Q9V2F3, large subunit polC: Q9V2F4 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus abyssi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | primer ssM13 DNA is the preferred substrate | Pyrococcus abyssi | ? | - |
? | |
| poly(dA)/oligo(dT)x + n dTTP | preferred substrate | Pyrococcus abyssi | poly(dA)/oligo(dT)x+n + n diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90000, SDS-PAGE | Pyrococcus abyssi |
| ? | x * 68100 (polB) + x * 139700 (polc), calculated from sequence | Pyrococcus abyssi |
| ? | x * 90000 (polB) + x * 140000 (polC), SDS-PAGE | Pyrococcus abyssi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA polymerase I | - |
Pyrococcus abyssi |
| DNA polymerase II | - |
Pyrococcus abyssi |
| Pol I | - |
Pyrococcus abyssi |
| Pol II | - |
Pyrococcus abyssi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the optimal temperature for polymerase activity could not be measured because activated DNA is not stable above 75°C | Pyrococcus abyssi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
potassium phosphate buffer | Pyrococcus abyssi |
| 8.5 | 9 | glycine/NaOH buffer | Pyrococcus abyssi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | pH 6.5: about 65% of maximal activity, pH 9.2: about 20% of maximal activity, potassium phosphate buffer | Pyrococcus abyssi |
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