Application | Comment | Organism |
---|---|---|
molecular biology | the high fidelty of the enzyme is suitable fo polymerase chain reaction (PCR), which requires accurate DNA amplification for gene cloning and diagnostic assay | Thermococcus waiotapuensis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Thermococcus waiotapuensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Thermococcus waiotapuensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
x * 90000, SDS-PAGE | Thermococcus waiotapuensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus waiotapuensis | H9CW54 | - |
- |
Thermococcus waiotapuensis DSM 12768 | H9CW54 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus waiotapuensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also posseses 3'->5' exonuclease activity | Thermococcus waiotapuensis | ? | - |
? | |
additional information | the enzyme also posseses 3'->5' exonuclease activity | Thermococcus waiotapuensis DSM 12768 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, SDS-PAGE | Thermococcus waiotapuensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermococcus waiotapuensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 85 | 60°C: about 65% of maximal activity, 85°C: about 75% of maximal activity | Thermococcus waiotapuensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
99 | - |
half-life: 4 h | Thermococcus waiotapuensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermococcus waiotapuensis |
7.5 | - |
assay at | Thermococcus waiotapuensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.5 | pH 5.5: about 50% of maximal activity, pH 6.5: about 50% of maximal activity | Thermococcus waiotapuensis |