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Literature summary for 2.7.7.7 extracted from

  • Zhang, L.; Lou, H.; Guo, L.; Zhan, Z.; Duan, Z.; Guo, X.; Huang, L.
    Accurate DNA synthesis by Sulfolobus solfataricus DNA polymerase B1 at high temperature (2010), Extremophiles, 14, 107-117.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
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additional information kinetic parameters for nucleotide incorporation by Sso pol B1 exo(-) at 55°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn approximately 2/3 of the errors made by the enzyme are single-base substitutions, of which 58% are C->T transition. Frameshift mutations, mostly resulting from single-base deletions, account for 19% of the total errors. An exonuclease-deficient mutant of Sso pol B1 is three times as mutagenic as the wild-type enzyme, suggesting that the intrinsic proofreading function contributed only modestly to the fidelity of the enzyme Saccharolobus solfataricus diphosphate + DNAn+1
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Synonyms

Synonyms Comment Organism
DNA polymerase B1
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Saccharolobus solfataricus
Sso pol B1
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Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
the replication fidelity of the enzyme is about three times as high at 70°C as at 55°C Saccharolobus solfataricus