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Literature summary for 2.7.7.7 extracted from
- The family X DNA polymerase from Deinococcus radiodurans adopts a non-standard extended conformation (2009), J. Biol. Chem., 284, 11992-11999.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Deinococcus radiodurans |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 0.1 M sodium acetate, pH 5, 0.2 M NaCl, 16% (w/v) 2-methyl-2,4-pentanedil | Deinococcus radiodurans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | the polymerase activity of PolX is strongly stimulated by Mn2+ | Deinococcus radiodurans |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus radiodurans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography and HiTrap heparin column chromatography | Deinococcus radiodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Deinococcus radiodurans | diphosphate + DNAn+1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA polymerase | - |
Deinococcus radiodurans |
| PolX | - |
Deinococcus radiodurans |
| X family DANN polymerase | - |
Deinococcus radiodurans |
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Release 2023.1 (January 2023)
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