Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Kirouac, K.N.; Ling, H.
    Structural basis of error-prone replication and stalling at a thymine base by human DNA polymerase iota (2009), EMBO J., 28, 1644-1654.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UNA4
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography and SP column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn DNA polymerase iota preferentially misincorporates nucleotides opposite thymines and halts replication at T bases Homo sapiens diphosphate + DNAn+1
-
?

Synonyms

Synonyms Comment Organism
DNA polymerase iota
-
Homo sapiens
poliota
-
Homo sapiens

General Information

General Information Comment Organism
physiological function poliota uniquely replicates DNA with a constrained active site, creating shorter C1'-C1' strand distances, with the finger domain projecting the template base out towards the solvent-accessible major groove and stabilizing a mismatched G base through H-bonding Homo sapiens