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Literature summary for 2.7.7.7 extracted from

  • DeCarlo, L.; Gowda, A.S.; Suo, Z.; Spratt, T.E.
    Formation of purine-purine mispairs by Sulfolobus solfataricus DNA polymerase IV (2008), Biochemistry, 47, 8157-8164.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
DTT
-
Saccharolobus solfataricus
Mg2+
-
Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn mechanism of purine-purine mispair formation, substrate specificity and binding structure, the kpol/Kd dNTP values for the insertion of dATP and dGTP opposite 7-deazaadenine and 7-deazaguanine are decreased over 10fold with respect to those of the unmodified nucleotides during formation of purine-purine mispairs. In addition, the rate of incorporation of 1-deaza-dATP opposite guanine is decreased 5fold. Dpo4 holds the incoming dNTP in the normal anti conformation while allowing the template nucleotide to change conformations to allow reaction to occur. This result may be functionally relevant in the replication of damaged DNA in that the polymerase may allow the template to adopt multiple configurations, overview Saccharolobus solfataricus diphosphate + DNAn+1
-
?
additional information potential structures of purine-purine base pairs, overview Saccharolobus solfataricus ?
-
?

Synonyms

Synonyms Comment Organism
DNA polymerase IV
-
Saccharolobus solfataricus
More the enzyme belongs to the Y-family polymerases and is a member of the RAD30A subfamily Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Saccharolobus solfataricus