Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | transcription from the promoter of the dinB gene is not significantly influenced by the DNA damage-inducing agent mitomycin C, thus mechanisms different from the classical RecA-dependent SOS response elevate Pol IV-dependent mutagenesis in starving Pseudomonas putida cells | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of Pol IV-defective dinB knockout mutants | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
identical to strain KT2440, gene dinB | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
measurement of mutagenesis frequency, overview | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme causes 1-bp deletions and different base substitutions in plasmids pKTpheA56+A and pKTpheA22TAG, pKTpheA22TAA, and pKTpheA22TGA, respectively, in stationary cells, overview, Pol IV-dependent mutagenesis causes an approximately 10fold increase in the frequency of accumulation of 1-bp deletion mutations on selective plates in wild-type populations starved for more than 1 week, development of a mutant detection assay system allowing to separately detect the mutants, no effect of Pol IV on the frequency of accumulation of base substitution mutations in starving cells is observed, overview, RecA independence of Pol IV-associated mutagenesis mechanisms different from the classical RecA-dependent SOS response can elevate Pol IV-dependent mutagenesis in starving cells, overview | Pseudomonas putida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase IV | - |
Pseudomonas putida |
Pol IV | - |
Pseudomonas putida |