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Literature summary for 2.7.7.7 extracted from

  • Tegova, R.; Tover, A.; Tarassova, K.; Tark, M.; Kivisaar, M.
    Involvement of error-prone DNA polymerase IV in stationary-phase mutagenesis in Pseudomonas putida (2004), J. Bacteriol., 186, 2735-2744.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information transcription from the promoter of the dinB gene is not significantly influenced by the DNA damage-inducing agent mitomycin C, thus mechanisms different from the classical RecA-dependent SOS response elevate Pol IV-dependent mutagenesis in starving Pseudomonas putida cells Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information construction of Pol IV-defective dinB knockout mutants Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
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identical to strain KT2440, gene dinB
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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measurement of mutagenesis frequency, overview Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme causes 1-bp deletions and different base substitutions in plasmids pKTpheA56+A and pKTpheA22TAG, pKTpheA22TAA, and pKTpheA22TGA, respectively, in stationary cells, overview, Pol IV-dependent mutagenesis causes an approximately 10fold increase in the frequency of accumulation of 1-bp deletion mutations on selective plates in wild-type populations starved for more than 1 week, development of a mutant detection assay system allowing to separately detect the mutants, no effect of Pol IV on the frequency of accumulation of base substitution mutations in starving cells is observed, overview, RecA independence of Pol IV-associated mutagenesis mechanisms different from the classical RecA-dependent SOS response can elevate Pol IV-dependent mutagenesis in starving cells, overview Pseudomonas putida ?
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Synonyms

Synonyms Comment Organism
DNA polymerase IV
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Pseudomonas putida
Pol IV
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Pseudomonas putida