Protein Variants | Comment | Organism |
---|---|---|
F12A | mutant enzyme shows disproportionately reduced activity on the damaged template | Escherichia coli |
F13V | mutant enzyme is almost unable to carry out translesion synthesis over N2-furfuryl-dG, although its activity on undamaged DNA is unaffected. The F13V mutation has a modest effect on the ability of DinB to discriminate against ribonucleotides, increasing the frequency of their misincorporation from less than 0.00001 to 0.001 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | wild-type DinB inserts deoxycytidine opposite N2-furfuryl-dG with 1015fold greater catalytic proficiency than opposite undamaged dG | Escherichia coli | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DinB DNA polymerase | - |
Escherichia coli |
DNA polymerase IV | - |
Escherichia coli |