Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | peak activity in the presence of Mg2+ is observed in the range of 0.1-0.5 mM and is significantly reduced at concentrations above 2 mM | Homo sapiens | |
Mn2+ | DNA polymerase iota exhibits the greatest activity in the presence of low levels of Mn2+ (0.05-0.25 mM). Mn2+ increases the catalytic activity of DNA polymerase iota by about 30000-60000fold through a strong decrease in the Km value for nucleotide incorporation. Whereas DNA polymerase iota preferentially misinserts G opposite T by a factor of about 1.4-2.5fold over the correct base A in the presence of 0.25 and 5 mM Mg2+, respectively, the correct insertion of A is actually favored 2fold over the misincorporation of guanine in the presence of 0.075 mM Mn2+. Low levels of Mn2+ also dramatically increase the ability of DNA polymerase iota to traverse a variety of DNA lesions in vitro. The cation utilized by DNA polymerase iota in vivo may be Mn2+ | Homo sapiens |
Organism | UniProt | Comment | Textmining |
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Homo sapiens | - |
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Synonyms | Comment | Organism |
---|---|---|
DNA polymerase iota | - |
Homo sapiens |