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Literature summary for 2.7.7.7 extracted from

  • Lee, Y.; Choi, J.J.; Kwon, S.
    Cloning, expression, and partial characterization of a family B-type DNA polymerase from the hyperthermophilic crenarchaeon Sulfophobococcus zilligii (2006), Enzyme Microb. Technol., 38, 821-830.
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Search for more literature for 2.7.7.7

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin DNA polymerase is stabilized with an approximately 25% increase in enzyme activity Sulfophobococcus zilligii
Triton X-100 DNA polymerase is stabilized with an approximately 25% increase in enzyme activity Sulfophobococcus zilligii

Cloned(Commentary)

Cloned (Comment) Organism
DNA polymerase gene is expressed under the control of the T7lac promoter on the expression vector pET-28a(+) in Escherichia coli BL21-CodonPlus(DE3)-RIL Sulfophobococcus zilligii

General Stability

General Stability Organism
DNA polymerase is stabilized by bovine serum albumin and Triton X-100 Sulfophobococcus zilligii

Inhibitors

Inhibitors Comment Organism Structure
EDTA 2 mM, complete inhibition Sulfophobococcus zilligii
K+
-
 Sulfophobococcus zilligii
NH4+
-
 Sulfophobococcus zilligii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates, optimal concentration is 1.5-2.5 mM Sulfophobococcus zilligii
Mn2+ optimal Mn2+ concentration is 0.5 mM Sulfophobococcus zilligii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89500
-
 x * 89500, SDS-PAGE Sulfophobococcus zilligii
91268
-
 x * 91268, calculated from sequence Sulfophobococcus zilligii

Organism

Organism UniProt Comment Textmining
Sulfophobococcus zilligii Q29Y55
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Sulfophobococcus zilligii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0033
-
-
 Sulfophobococcus zilligii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn calf thymus DNA Sulfophobococcus zilligii diphosphate + DNAn+1
-
 ?
additional information Szi DNA polymerase possesses associated 3'-5' and 5'-3' exonuclease activities Sulfophobococcus zilligii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 89500, SDS-PAGE Sulfophobococcus zilligii
? x * 91268, calculated from sequence Sulfophobococcus zilligii

Synonyms

Synonyms Comment Organism
family B-type DNA polymerase
-
 Sulfophobococcus zilligii
Szi DNA polymerase
-
 Sulfophobococcus zilligii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 calf thymus DNA template Sulfophobococcus zilligii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 90 60°C: about 45% of maximal activity, 90°C: about 40% of maximal activity, calf thymus DNA template Sulfophobococcus zilligii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 half-life in presence of 0.02% bovine serum albumin: 4.5 h Sulfophobococcus zilligii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Sulfophobococcus zilligii