Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzyme R258A are expressed in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
R258A | | mutant of Pol beta has a facilitated subdomain-reopening step. Rate of single-nucleotide incorporation catalyzed by R258A is identical to that of wild-type | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
African swine fever virus | - |
- |
- |
Escherichia coli | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | a fast fluorescence transition corresponding to conformational closing, and a slow fluorescence transition matching the rate of single-nucleotide incorporation. This transition represents a conformational event after chemistry, likely subdomain reopening | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | a fast fluorescence transition corresponding to conformational closing, and a slow fluorescence transition matching the rate of single-nucleotide incorporation. This transition represents a conformational event after chemistry, likely subdomain reopening | African swine fever virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | a fast fluorescence transition corresponding to conformational closing, and a slow fluorescence transition matching the rate of single-nucleotide incorporation. This transition represents a conformational event after chemistry, likely subdomain reopening. Rotation of the Arg258 side chain is not rate-limiting in the overall kinetic pathway of Pol beta, yet is kinetically significant in subdomain reopening | Rattus norvegicus | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase beta | - |
Rattus norvegicus |
DNA polymerase I | Klenow fragment | Escherichia coli |
DNA polymerase X | - |
African swine fever virus |
Klenow fragment | of DNA polymerase I | Escherichia coli |
Pol X | - |
African swine fever virus |