Literature summary for 2.7.7.7 extracted from

Villbrandt, B.; Sagner, G.; Schomburg, D.
Investigations on the thermostability and function of truncated Thermus aquaticus DNA polymerase fragments (1997), Protein Eng., 10, 1281-1288.

Search for more literature for 2.7.7.7

Application

Application	Comment	Organism
biotechnology	thermostable polymerase used in PCR	Thermus aquaticus
molecular biology	thermostable polymerase used in PCR	Thermus aquaticus

Cloned(Commentary)

Cloned (Comment)	Organism
truncated versions lacking 288,422 and 413 amino acids at the N-terminus, fusion protein with beta-galactosidase	Thermus aquaticus

Protein Variants

Protein Variants	Comment	Organism
L831N/A814R	truncated enzyme delta413-L813N/A814R has reduced temperature stability	Thermus aquaticus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
deoxynucleoside triphosphate + DNAn	Thermus aquaticus	-	diphosphate + DNAn+1	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermus aquaticus	P19821	-	-

Purification (Commentary)

Purification (Comment)	Organism
truncated enzyme	Thermus aquaticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
deoxynucleoside triphosphate + DNAn	-	Thermus aquaticus	diphosphate + DNAn+1	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	71% wild-type enzyme activity after 5 min, 8% delta413 mutant enzyme activity remains after 5 min, 32% delta422 mutant enzyme activity after 5 min, 50% delta413-L813N/A814R mutant enzyme activity after 5 min	Thermus aquaticus
95	-	61% wild-type enzyme activity after 5 min, no delta413 mutant enzyme activity remains after 5 min, 11% delta422 mutant enzyme activity after 5 min, 20% delta413-L813N/A814R mutant enzyme activity after 5 min	Thermus aquaticus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)