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Literature summary for 2.7.7.7 extracted from

  • Park, J.H.; Kim, J.S.; Kwon, S.T.; Lee, D.S.
    Purification and characterization of Thermus caldophilus GK24 DNA polymerase (1993), Eur. J. Biochem., 214, 135-140.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration: 12 mM Thermus caldophilus
Mn2+ can partially replace Mg2+ in activation Thermus caldophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
gel filtration, SDS-PAGE Thermus caldophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Thermus caldophilus
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Thermus caldophilus GH24
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Thermus caldophilus
-
-
-
Thermus caldophilus GH24
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus caldophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
28
-
-
Thermus caldophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Thermus caldophilus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn exonuclease 5'--3' activity Thermus caldophilus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn no exonuclease 3'--5' activity Thermus caldophilus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Thermus caldophilus GH24 diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn exonuclease 5'--3' activity Thermus caldophilus GH24 diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn no exonuclease 3'--5' activity Thermus caldophilus GH24 diphosphate + DNAn+1
-
?

Subunits

Subunits Comment Organism
monomer 1 * 95000, SDS-PAGE Thermus caldophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Thermus caldophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
70 min, 50% inactivation Thermus caldophilus
100
-
5 min, 50% inactivation Thermus caldophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
-
Thermus caldophilus

pH Range

pH Minimum pH Maximum Comment Organism
8.5 8.7 narrow pH range Thermus caldophilus

pI Value

Organism Comment pI Value Maximum pI Value
Thermus caldophilus
-
-
6.5