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Literature summary for 2.7.7.7 extracted from

  • Pluthero, F.G.
    Rapid purification of high-activity Taq DNA polymerase (1993), Nucleic Acids Res., 21, 4850-4851.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology thermostable polymerase used in PCR Thermus aquaticus
molecular biology thermostable polymerase used in PCR Thermus aquaticus

Cloned(Commentary)

Cloned (Comment) Organism
-
Thermus aquaticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Thermus aquaticus
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Thermus aquaticus INValphaF'
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
Escherichia coli transformed with the pTaq plasmid containing the Taq gene expressed under control of the tac promoter
-
Thermus aquaticus INValphaF'
-
Escherichia coli transformed with the pTaq plasmid containing the Taq gene expressed under control of the tac promoter
-

Purification (Commentary)

Purification (Comment) Organism
strain INValphaF' of Escherichia coli transformed with the pTaq plasmid containing the Taq gene expressed under control of the tac promoter Thermus aquaticus

Storage Stability

Storage Stability Organism
-20°C, stable over 12 months -70°C, stable over 18 months Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Thermus aquaticus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Thermus aquaticus INValphaF' diphosphate + DNAn+1
-
?