General Stability | Organism |
---|---|
sucrose, dextrose and glycerol stabilize | Strongylocentrotus purpuratus |
sucrose, dextrose and glycerol stabilize | Mesocentrotus franciscanus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Mesocentrotus franciscanus | |
ethanol | 3%, 50% inhibition | Mesocentrotus franciscanus | |
Sodium dodecyl sulfate | - |
Mesocentrotus franciscanus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | divalent cation required, Mg2+ or Mn2+ | Strongylocentrotus purpuratus | |
Mg2+ | divalent cation required, Mg2+ or Mn2+ | Mesocentrotus franciscanus | |
Mg2+ | stimulates at 9 mM | Mesocentrotus franciscanus | |
Mn2+ | stimulates at 0.5 mM, about a third the maximal activity with Mg2+ | Mesocentrotus franciscanus | |
Mn2+ | divalent cation required, Mn2+ or Mg2+ | Strongylocentrotus purpuratus | |
Mn2+ | divalent cation required, Mn2+ or Mg2+ | Mesocentrotus franciscanus | |
Zn2+ | 10-13% of the activity with Mg2+, optimal concentration: 0.3-0.5 mM | Mesocentrotus franciscanus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | 168000 | gel filtration, glycerol gradient centrifugation | Mesocentrotus franciscanus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Strongylocentrotus purpuratus | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Mesocentrotus franciscanus | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesocentrotus franciscanus | - |
sea urchin | - |
Strongylocentrotus purpuratus | - |
sea urchin | - |
Purification (Comment) | Organism |
---|---|
- |
Mesocentrotus franciscanus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Strongylocentrotus purpuratus | - |
embryo | - |
Mesocentrotus franciscanus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.027 | - |
- |
Strongylocentrotus purpuratus |
0.027 | - |
- |
Mesocentrotus franciscanus |
Storage Stability | Organism |
---|---|
-70°C, 20% glycerol, 20 mM postassium phosphate, 4 mM reduced glutathione, 1 mM EDTA, stable for 6 years | Strongylocentrotus purpuratus |
-70°C, 20% glycerol, 20 mM postassium phosphate, 4 mM reduced glutathione, 1 mM EDTA, stable for 6 years | Mesocentrotus franciscanus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Strongylocentrotus purpuratus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Mesocentrotus franciscanus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | template specificity | Mesocentrotus franciscanus | diphosphate + DNAn+1 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.2 | - |
Strongylocentrotus purpuratus |
7.8 | 8.2 | - |
Mesocentrotus franciscanus |