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Literature summary for 2.7.7.7 extracted from

  • Fansler, B.S.; Loeb, L.A.
    Sea urchin nuclear DNA polymerase (1974), Methods Enzymol., 29, 53-70.
    View publication on PubMed

General Stability

General Stability Organism
sucrose, dextrose and glycerol stabilize Strongylocentrotus purpuratus
sucrose, dextrose and glycerol stabilize Mesocentrotus franciscanus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Mesocentrotus franciscanus
ethanol 3%, 50% inhibition Mesocentrotus franciscanus
Sodium dodecyl sulfate
-
Mesocentrotus franciscanus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation required, Mg2+ or Mn2+ Strongylocentrotus purpuratus
Mg2+ divalent cation required, Mg2+ or Mn2+ Mesocentrotus franciscanus
Mg2+ stimulates at 9 mM Mesocentrotus franciscanus
Mn2+ stimulates at 0.5 mM, about a third the maximal activity with Mg2+ Mesocentrotus franciscanus
Mn2+ divalent cation required, Mn2+ or Mg2+ Strongylocentrotus purpuratus
Mn2+ divalent cation required, Mn2+ or Mg2+ Mesocentrotus franciscanus
Zn2+ 10-13% of the activity with Mg2+, optimal concentration: 0.3-0.5 mM Mesocentrotus franciscanus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000 168000 gel filtration, glycerol gradient centrifugation Mesocentrotus franciscanus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Strongylocentrotus purpuratus
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Mesocentrotus franciscanus
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Mesocentrotus franciscanus
-
sea urchin
-
Strongylocentrotus purpuratus
-
sea urchin
-

Purification (Commentary)

Purification (Comment) Organism
-
Mesocentrotus franciscanus

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Strongylocentrotus purpuratus
-
embryo
-
Mesocentrotus franciscanus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.027
-
-
Strongylocentrotus purpuratus
0.027
-
-
Mesocentrotus franciscanus

Storage Stability

Storage Stability Organism
-70°C, 20% glycerol, 20 mM postassium phosphate, 4 mM reduced glutathione, 1 mM EDTA, stable for 6 years Strongylocentrotus purpuratus
-70°C, 20% glycerol, 20 mM postassium phosphate, 4 mM reduced glutathione, 1 mM EDTA, stable for 6 years Mesocentrotus franciscanus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Strongylocentrotus purpuratus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Mesocentrotus franciscanus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn template specificity Mesocentrotus franciscanus diphosphate + DNAn+1
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8.2
-
Strongylocentrotus purpuratus
7.8 8.2
-
Mesocentrotus franciscanus