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Literature summary for 2.7.7.7 extracted from

  • Wei, X.; Peterson, D.L.
    Expression, purification and characterization of an active RNase H domain of the Hepatitis B viral polymerase (1996), J. Biol. Chem., 271, 32617-32622.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
RNase H domain Hepatitis B virus

Inhibitors

Inhibitors Comment Organism Structure
KCl inhibition above 20 mM Hepatitis B virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ enzyme prefers Mg2+ over Mn2+ Hepatitis B virus
Mn2+ enzyme prefers Mg2+ over Mn2+ Hepatitis B virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19960
-
calculated from amino acid composition, RNase H domain Hepatitis B virus
20000
-
RNase H domain, SDS-PAGE Hepatitis B virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Hepatitis B virus
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Hepatitis B virus P03156
-
-

Oxidation Stability

Oxidation Stability Organism
sensitive to oxidation, enzyme can be stabilized by addition of dithiothreitol or 2-mercaptoethanol Hepatitis B virus

Purification (Commentary)

Purification (Comment) Organism
RNase H domain Hepatitis B virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Hepatitis B virus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn RNase H domain degrades RNA component of RNA-DNA hybrids Hepatitis B virus diphosphate + DNAn+1
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable for 10 min, RNase H activity Hepatitis B virus
100
-
no RNase H activity after 10 min Hepatitis B virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Hepatitis B virus