Cloned (Comment) | Organism |
---|---|
RNase H domain | Hepatitis B virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | inhibition above 20 mM | Hepatitis B virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | enzyme prefers Mg2+ over Mn2+ | Hepatitis B virus | |
Mn2+ | enzyme prefers Mg2+ over Mn2+ | Hepatitis B virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19960 | - |
calculated from amino acid composition, RNase H domain | Hepatitis B virus |
20000 | - |
RNase H domain, SDS-PAGE | Hepatitis B virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Hepatitis B virus | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepatitis B virus | P03156 | - |
- |
Oxidation Stability | Organism |
---|---|
sensitive to oxidation, enzyme can be stabilized by addition of dithiothreitol or 2-mercaptoethanol | Hepatitis B virus |
Purification (Comment) | Organism |
---|---|
RNase H domain | Hepatitis B virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Hepatitis B virus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | RNase H domain degrades RNA component of RNA-DNA hybrids | Hepatitis B virus | diphosphate + DNAn+1 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for 10 min, RNase H activity | Hepatitis B virus |
100 | - |
no RNase H activity after 10 min | Hepatitis B virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Hepatitis B virus |