Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
(2S)-2-phospholactate | pH 7.5, 70°C | Methanocaldococcus jannaschii | |
0.056 | - |
GTP | pH 7.5, 70°C | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, 16fold activation | Methanocaldococcus jannaschii | |
Fe2+ | 1 mM, 10fold activation | Methanocaldococcus jannaschii | |
Mg2+ | 1 mM, 23fold activation | Methanocaldococcus jannaschii | |
Mn2+ | 1 mM, 6fold activation | Methanocaldococcus jannaschii | |
Ni2+ | 1 mM, 8fold activation | Methanocaldococcus jannaschii | |
Zn2+ | 1 mM, 3fold activation | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
2 * 25000, recombinant enzyme, SDS-PAGE | Methanocaldococcus jannaschii |
50000 | - |
recombinant enzyme, gel filtration | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-phospholactate + GTP | Methanocaldococcus jannaschii | third step of coenzyme F420 biosynthesis | lactyl-2-diphospho-5'-guanosine + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58297 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
due to the instability of the product lactyl-2-diphospho-5'-guanosine, the reaction is monitored indirectly through a coupled assay with 7,8-didemethyl-8-hydroxy-5-deazaflavin catalyzed by 2-phospho-L-lactate transferase (CofD, MJ1256) to form coenzyme F420-0 | Methanocaldococcus jannaschii |
1.3 | - |
assay with gamma-thioGTP | Methanocaldococcus jannaschii |
2.3 | - |
assay with ITP | Methanocaldococcus jannaschii |
3.3 | - |
assay with dGTP | Methanocaldococcus jannaschii |
3.5 | - |
assay with GTP | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-2-phospholactate + dGTP | specific activity is about 95% of the activity with GTP | Methanocaldococcus jannaschii | (2S)-lactyl-2-diphospho-5'-(2'-deoxy)guanosine + diphosphate | - |
? | |
(2S)-2-phospholactate + gamma-thiolGTP | specific activity is about 65% of the activity with GTP | Methanocaldococcus jannaschii | ? | - |
? | |
(2S)-2-phospholactate + GTP | - |
Methanocaldococcus jannaschii | lactyl-2-diphospho-5'-guanosine + diphosphate | - |
? | |
(2S)-2-phospholactate + GTP | third step of coenzyme F420 biosynthesis | Methanocaldococcus jannaschii | lactyl-2-diphospho-5'-guanosine + diphosphate | - |
? | |
(2S)-2-phospholactate + ITP | specific activity is about 35% of the activity with GTP | Methanocaldococcus jannaschii | lactyl-2-diphospho-5'-inosine + diphosphate + diphosphate | - |
? | |
additional information | weak activity with GDP and ATP | Methanocaldococcus jannaschii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 25000, recombinant enzyme, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
cofC | - |
Methanocaldococcus jannaschii |
MJ0887 | - |
Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
10 min, completely stable | Methanocaldococcus jannaschii |
100 | - |
10 min, 62% loss of activity | Methanocaldococcus jannaschii |