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Literature summary for 2.7.7.66 extracted from

  • Hoenke, S.; Wild, M.R.; Dimroth, P.
    Biosynthesis of triphosphoribosyl-dephospho-coenzyme A, the precursor of the prosthetic group of malonate decarboxylase (2000), Biochemistry, 39, 13223-13232.
    View publication on PubMed
Search for more literature for 2.7.7.66

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
D134A mutation abolishes the transfer of the prosthetic group to apo acyl carrier protein, but the binding of triphosphoribosyl-dephospho-CoA to MdcG is not affected Klebsiella pneumoniae
D134A/D136A mutation abolishes the transfer of the prosthetic group to apo acyl carrier protein, but the binding of triphosphoribosyl-dephospho-CoA to MdcG is not affected Klebsiella pneumoniae
D136A mutation abolishes the transfer of the prosthetic group to apo acyl carrier protein, but the binding of triphosphoribosyl-dephospho-CoA to MdcG is not affected Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-(5''-triphosphoribosyl)-3'-dephospho-CoA + malonate decarboxylase apo-[acyl-carrier protein]
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 Klebsiella pneumoniae malonate decarboxylase holo-[acyl-carrier protein] + diphosphate
-
 ?
2'-(5''-triphosphoribosyl)-3'-dephospho-CoA + malonate decarboxylase apo-[acyl-carrier protein] MdcG forms a strong complex with the 2'-(5''-triphosphoribosyl)-3'-dephospho-CoA prosthetic group precursor. This complex is called MdcGi. Upon incubation of MdcGi with apo acyl carrier protein, holo acyl carrier protein is synthesized by forming the phosphodiester bond between the 2'-(5''-phosphoribosyl)-3'-dephospho-CoA prosthetic group and Ser25 of the protein. In absence of the prosthetic group precursor, MdcG catalyzes at a low rate the adenylylation of apo acyl carrier protein using ATP as substrate. The adenylyl ACP thus formed is an unphysiological side product and is not involved in the biosynthesis of holo ACP Klebsiella pneumoniae malonate decarboxylase holo-[acyl-carrier protein] + diphosphate
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 ?

Synonyms

Synonyms Comment Organism
2'-(5''-triphosphoribosyl)-3'-dephospho-CoA:apo ACP 2'-(5''-phosphoribosyl)-3'-dephospho-CoA transferase
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 Klebsiella pneumoniae
MdcG
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 Klebsiella pneumoniae