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Literature summary for 2.7.7.65 extracted from
- A crosstalk between c-di-GMP and cAMP in regulating transcription of GcsA, a diguanylate cyclase involved in swimming motility in Pseudomonas putida (2020), Environ. Microbiol., 22, 142-157 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Pseudomonas putida has dozens of DGC/PDE-encoding genes in its genome, identification of the transcription start point of gcsA, cloning of the promoter of gcsA | Pseudomonas putida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a gcsA-deletion mutant. The deletion and complementation of gcsA shows no obvious influence on the growth rate of the strains in liquid LB broth. The normalized GFP fluorescence in the gcsA-deletion strain and complemented strain shows no significant difference compared to that in the wild-type strain at 24 h after inoculation. The gcsA deletion mutant contains about a 27% lower amount of c-di-GMP, and the gcsA-complemented strain contains about a 30% higher amount of c-di-GMP compared to the wild-type strain at the 5 h time point. Comparison of swimming motility, biofilm formation, and surface morphology of the gcsA mutant and complemented strain, overview | Pseudomonas putida |
| additional information | mutation in the GGDEF motif from GGDEF to GGAAF abolishes the cyclic diguanylate synthesis ability of GcsA | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 GTP | Pseudomonas putida | - |
2 diphosphate + cyclic di-3',5'-guanylate | - |
? |  |
| 2 GTP | Pseudomonas putida DSM 6125 | - |
2 diphosphate + cyclic di-3',5'-guanylate | - |
? | |
| 2 GTP | Pseudomonas putida NCIMB 11950 | - |
2 diphosphate + cyclic di-3',5'-guanylate | - |
? | |
| 2 GTP | Pseudomonas putida ATCC 47054 | - |
2 diphosphate + cyclic di-3',5'-guanylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida | Q88GL0 | - |
- |
| Pseudomonas putida ATCC 47054 | Q88GL0 | - |
- |
| Pseudomonas putida DSM 6125 | - |
- |
- |
| Pseudomonas putida DSM 6125 | Q88GL0 | - |
- |
| Pseudomonas putida NCIMB 11950 | Q88GL0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 GTP | - |
Pseudomonas putida | 2 diphosphate + cyclic di-3',5'-guanylate | - |
? | |
| 2 GTP | - |
Pseudomonas putida DSM 6125 | 2 diphosphate + cyclic di-3',5'-guanylate | - |
? | |
| 2 GTP | - |
Pseudomonas putida NCIMB 11950 | 2 diphosphate + cyclic di-3',5'-guanylate | - |
? | |
| 2 GTP | - |
Pseudomonas putida ATCC 47054 | 2 diphosphate + cyclic di-3',5'-guanylate | - |
? | |
| additional information | GcsA has no PDE activity | Pseudomonas putida | ? | - |
- |
|
| additional information | GcsA has no PDE activity | Pseudomonas putida DSM 6125 | ? | - |
- |
|
| additional information | GcsA has no PDE activity | Pseudomonas putida NCIMB 11950 | ? | - |
- |
|
| additional information | GcsA has no PDE activity | Pseudomonas putida ATCC 47054 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | enzyme GcsA consists of two per-ARNT-sim (PAS) domains, followed by a canonical conserved central sequence pattern (GGDEF) domain and a truncated EAL domain | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GcsA | - |
Pseudomonas putida |
| PP_3711 | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Pseudomonas putida |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Pseudomonas putida | crosstalk between c-di-GMP and cAMP in the regulation of the expression of GcsA in Pseudomonas putida. In terms of transcriptional regulation, gcsA is cooperatively regulated by c-di-GMP and cAMP via their effectors, FleQ and Crp respectively. The transcription of gcsA is inhibited by cAMP. Crp directly regulated the transcription of gcsA by binding to its promoter. cAMP regulates expression of gcsA via Crp, a catabolite activator protein, that binds to the gcsA promoter DNA | down |
| Pseudomonas putida | FleQ indirectly regulated the transcription of gcsA, while Crp directly regulated the transcription of gcsA by binding to its promoter. c-di-GMP and cAMP cooperatively regulate the expression of gcsA | additional information |
| Pseudomonas putida | transcription of gcsA is cooperatively regulated by c-di-GMP and cAMP via their effectors, FleQ and Crp respectively | additional information |
| Pseudomonas putida | crosstalk between c-di-GMP and cAMP in the regulation of the expression of GcsA in Pseudomonas putida. In terms of transcriptional regulation, gcsA is cooperatively regulated by c-di-GMP and cAMP via their effectors, FleQ and Crp respectively. The transcription of gcsA is promoted by c-di-GMP. FleQ indirectly regulated the transcription of gcsA. di-GMP regulates the expression of gcsA via FleQ, an enhancer binding protein of the NtrC family of transcriptional regulators that binds c-di-GMP | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | Pseudomonas putida strain KT2440 has dozens of DGC/PDE-encoding genes in its genome. GcsA homologues are also found in strains of several other Pseudomonas species, including Pseudomonas monteilii strain SB3078, Pseudomonas plecoglossicida strain NyZ12, and Pseudomonas cremoricolorata strain ND07, which share more than 79% amino acid sequence identity with one another, but no GcsA homologues are found in the model organisms Pseudomonas aeruginosa strains PAO1 and PA14, Pseudomonas fluorescens strain Pfl5, and Pseudomonas syringae pv. tomato strain DC3000 | Pseudomonas putida |
| malfunction | mutation in the GGDEF motif (from GGDEF to GGAAF) abolishes the c-di-GMP synthesis ability of GcsA | Pseudomonas putida |
| physiological function | diguanylate cyclase (DGC) synthesizes the ubiquitous bacterial second messenger c-di-GMP. GcsA has no PDE activity. GcsA modulates the cellular c-di-GMP level at early exponential phase. Crosstalk between c-di-GMP and cAMP in the regulation of the expression of GcsA in Pseudomonas putida. GcsA plays a role in early biofilm formation of Pseudomonas putida and regulates the swimming motility in an FlgZ-dependent manner | Pseudomonas putida |
| physiological function | GcsA inhibits swimming motility in an FlgZ-dependent manner. The GGDEF domain of GcsA contains all the conserved signature amino acid residues that form the enzyme's active site, as well as other residues required for the diguanylate cyclase activity of the GGDEF domain. GcsA modulates the cellular cyclic di-GMP level at early exponential phase | Pseudomonas putida |
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