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Literature summary for 2.7.7.65 extracted from

  • Poudyal, B.; Sauer, K.
    The PA3177 gene encodes an active diguanylate cyclase that contributes to biofilm antimicrobial tolerance but not biofilm formation by Pseudomonas aeruginosa (2018), Antimicrob. Agents Chemother., 62, e01049-18 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.65

Cloned(Commentary)

Cloned (Comment) Organism
gene PA3177, quantitative reverse transcription-PCR enzyme expression analysis Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information construction of DELTAPPA3177 deletion strain, phenotype, overview Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 GTP Pseudomonas aeruginosa
-
 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP Pseudomonas aeruginosa ATCC 15692
-
 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP Pseudomonas aeruginosa 1C
-
 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP Pseudomonas aeruginosa PRS 101
-
 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP Pseudomonas aeruginosa DSM 22644
-
 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP Pseudomonas aeruginosa CIP 104116
-
 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP Pseudomonas aeruginosa LMG 12228
-
 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP Pseudomonas aeruginosa JCM 14847
-
 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HZ57
-
-
Pseudomonas aeruginosa 1C Q9HZ57
-
-
Pseudomonas aeruginosa ATCC 15692 Q9HZ57
-
-
Pseudomonas aeruginosa CIP 104116 Q9HZ57
-
-
Pseudomonas aeruginosa DSM 22644 Q9HZ57
-
-
Pseudomonas aeruginosa JCM 14847 Q9HZ57
-
-
Pseudomonas aeruginosa LMG 12228 Q9HZ57
-
-
Pseudomonas aeruginosa PRS 101 Q9HZ57
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information quantitative reverse transcription-PCR (qRT-PCR) confirms the increase in transcript abundance of PA3177 to be 6.5fold in biofilm cells relative to planktonic cells Pseudomonas aeruginosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 GTP
-
 Pseudomonas aeruginosa 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP
-
 Pseudomonas aeruginosa ATCC 15692 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP
-
 Pseudomonas aeruginosa 1C 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP
-
 Pseudomonas aeruginosa PRS 101 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP
-
 Pseudomonas aeruginosa DSM 22644 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP
-
 Pseudomonas aeruginosa CIP 104116 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP
-
 Pseudomonas aeruginosa LMG 12228 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP
-
 Pseudomonas aeruginosa JCM 14847 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?

Synonyms

Synonyms Comment Organism
GGDEF domain-containing protein UniProt Pseudomonas aeruginosa
PA3177
-
 Pseudomonas aeruginosa

General Information

General Information Comment Organism
malfunction inactivation of PA3177 renders Pseudomonas aeruginosa strain PAO1 biofilms susceptible to tobramycin and hydrogen peroxide. Inactivation of PA3177 also eliminates the recalcitrance of biofilms to killing by tobramycin, with multicopy expression of PA3177 but not PA3177_GGAAF harboring substitutions in the active site, restoring tolerance to wild-type levels. While PA3177 contributes to biofilm drug tolerance, inactivation of PA3177 has no effect on attachment and biofilm formation. Inactivation of PA3177 correlates with reduced c-di-GMP levels in biofilm but not planktonic cells. Inactivation of PA3177 has no effect on attachment and the formation of mature biofilms characterized by a three-dimensional biofilm architecture Pseudomonas aeruginosa
metabolism BrlR is a c-di-GMP responsive transcriptional regulator. Inactivation of PA3177 coincides with BrlR abundance being reduced in biofilms and unable to bind to PmexE, inactivation of PA3177 coincides with reduced BrlR protein abundance Pseudomonas aeruginosa
physiological function PA3177 is confirmed to be an active diguanylate cyclase, with overexpression affecting swimming and swarming motility, and inactivation affecting cellular bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) levels of biofilm but not planktonic cells. PA3177 is confirmed to be an active diguanylate cyclase, with overexpression affecting swimming and swarming motility, and inactivation affecting cellular c-di-GMP levels of biofilm but not planktonic cells. While PA3177 contributes to biofilm drug tolerance, inactivation of PA3177 has no effect on attachment and biofilm formation Pseudomonas aeruginosa