Protein Variants | Comment | Organism |
---|---|---|
D70A | mutated in the response regulator domain, no significant formation of clusters | Pseudomonas aeruginosa |
E253A | mutated in active site, protein forms subcellular clusters in broth-grown cells | Pseudomonas aeruginosa |
L167D | mutated in linker stalk, low formation of clusters | Pseudomonas aeruginosa |
L170D | mutated in linker stalk, protein forms subcellular clusters in broth-grown cells | Pseudomonas aeruginosa |
R198A | mutated in I site, protein forms subcellular clusters in broth-grown cells | Pseudomonas aeruginosa |
V72D | mutated in the response regulator domain, no significant formation of clusters | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HXT9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | when activated by phosphorylation, yellow fluorescent protein (YFP)-tagged WspR forms clusters that are visible in individual cells by fluorescence microscopy. Unphosphorylated WspR is diffuse in cells and not visible. In general, increased cluster formation correlates with increased in vivo and in vitro diguanylate cyclase activities of variants. In addition, WspR specific activity is strongly concentration dependent in vitro, and the effect of the protein concentration on diguanylate cyclase activity is magnified when WspR is treated with the phosphor analog beryllium fluoride. Cluster formation appears to be an intrinsic property of phosphorylated WspR | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
WspR | - |
Pseudomonas aeruginosa |