Protein Variants | Comment | Organism |
---|---|---|
K759A | mutagenesis of a conserved lysine residue, results in a severe reduction in dihuanylate cyclase activity, kcat value is almost 100fold lower than that of the wild-type protein while the K1 and K2 values do not change very much | Xanthomonas citri pv. citri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: cyclic di-GMP | Xanthomonas citri pv. citri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
GTP | mutant K759A, Michaelis-Menten model, pH 8.0, 30°C | Xanthomonas citri pv. citri | |
0.061 | - |
GTP | Michaelis-Menten model, pH 8.0, 30°C | Xanthomonas citri pv. citri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | or Mn2+, strictly required | Xanthomonas citri pv. citri | |
Mn2+ | or Mg2+, strictly required | Xanthomonas citri pv. citri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94500 | - |
- |
Xanthomonas citri pv. citri |
360000 | - |
gel filtration | Xanthomonas citri pv. citri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas citri pv. citri | Q8PPS5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 GTP | - |
Xanthomonas citri pv. citri | 2 diphosphate + cyclic di-3',5'-guanylate | - |
? | |
additional information | sequential random binding model of two identical substrates binding to two equivalent GGDEF domains that come together to form a symmetrical active site | Xanthomonas citri pv. citri | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | at micromolar concentrations, the protein exists predominantly as a dimeric species | Xanthomonas citri pv. citri |
multimer | x * 94500, calculated | Xanthomonas citri pv. citri |
Synonyms | Comment | Organism |
---|---|---|
XAC0610 | - |
Xanthomonas citri pv. citri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
GTP | mutant K759A, Michaelis-Menten model, pH 8.0, 30°C | Xanthomonas citri pv. citri | |
1.2 | - |
GTP | wild-type, Michaelis-Menten model, pH 8.0, 30°C | Xanthomonas citri pv. citri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Xanthomonas citri pv. citri |
General Information | Comment | Organism |
---|---|---|
physiological function | protein has in vivo and in vitro diguanylate cyclase activity that leads to the production of cyclic di-GMP. Protein XAC0610 plays a role in the regulation of Xanthomonas citri motility and resistance to H2O2. XAC0610 is not subject to allosteric product inhibition. Instead, steady-state kinetics reveal a positive cooperative effect of the GTP substrate with a dissociation constant for the binding of the first GTP molecule (K1) approximately 5 times greater than the dissociation constant for the binding of the second GTP molecule (K2). The N-terminal GAF and PAS domains are required for high levels of XAC0610 diguanylate cyclase activity | Xanthomonas citri pv. citri |