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Literature summary for 2.7.7.65 extracted from
- Interaction of the diguanylate cyclase YdeH of Escherichia coli with 2,(3)-substituted purine and pyrimidine nucleotides (2011), J. Pharmacol. Exp. Ther., 336, 234-241.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2',3'-O-[2,4,6-trinitrocyclohexa-2,5-diene-1,1-diyl]-GTP | - |
Escherichia coli |  |
| 2'-(N-methylanthraniloyl)-GTP | - |
Escherichia coli | |
| 2'-(N-methylanthraniloyl)-GTP-gamma-S | - |
Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | - |
Escherichia coli | cyclic di-3',5'-guanylate + diphosphate | - |
? | |
| additional information | the interaction between MANT (2'(3'))-O-(N-methylanthraniloyl)- or TNP (2',3'-O-(2,4,6-trinitrophenyl))-substituted nucleotides and the DGC YdeH by fluorimetric and mass-spectrometric means is investigated. A strong fluorescence resonance energy transfer between tryptophan and tyrosine residues of YdeH and the MANT group of MANT-NTPs (MANT-ATP,-CTP, -GTP, -ITP, -UTP, and -XTP) and an enhanced direct MANT fluorescence upon interaction with YdeH is observed | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| diguanylate cyclase | - |
Escherichia coli |
| YdeH | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
pH 8, 30°C | Escherichia coli | 2',3'-O-[2,4,6-trinitrocyclohexa-2,5-diene-1,1-diyl]-GTP | |
| 0.0002 | - |
pH 8, 30°C | Escherichia coli | 2'-(N-methylanthraniloyl)-GTP-gamma-S | |
| 0.0005 | - |
pH 8, 30°C | Escherichia coli | 2'-(N-methylanthraniloyl)-GTP | |
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Release 2023.1 (January 2023)
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